Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity

• There is a growing interest in epigenetic modifications, and more specifically in histone post-translational modifications (hPTMs). • We describe an MS-based workflow that is capable of tracking all possible hPTMs in an untargeted approach that makes use of human cells. • Improving the workflow an...

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Detalles Bibliográficos
Autores principales: Verhelst, Sigrid, De Clerck, Laura, Willems, Sander, Van Puyvelde, Bart, Daled, Simon, Deforce, Dieter, Dhaenens, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508989/
https://www.ncbi.nlm.nih.gov/pubmed/32995308
http://dx.doi.org/10.1016/j.mex.2020.101055
Descripción
Sumario:• There is a growing interest in epigenetic modifications, and more specifically in histone post-translational modifications (hPTMs). • We describe an MS-based workflow that is capable of tracking all possible hPTMs in an untargeted approach that makes use of human cells. • Improving the workflow and the user-friendliness in order to become a high throughput, easily applicable, toxicological screening assay is an ongoing effort.

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