Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity

• There is a growing interest in epigenetic modifications, and more specifically in histone post-translational modifications (hPTMs). • We describe an MS-based workflow that is capable of tracking all possible hPTMs in an untargeted approach that makes use of human cells. • Improving the workflow an...

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Autores principales: Verhelst, Sigrid, De Clerck, Laura, Willems, Sander, Van Puyvelde, Bart, Daled, Simon, Deforce, Dieter, Dhaenens, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508989/
https://www.ncbi.nlm.nih.gov/pubmed/32995308
http://dx.doi.org/10.1016/j.mex.2020.101055
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author Verhelst, Sigrid
De Clerck, Laura
Willems, Sander
Van Puyvelde, Bart
Daled, Simon
Deforce, Dieter
Dhaenens, Maarten
author_facet Verhelst, Sigrid
De Clerck, Laura
Willems, Sander
Van Puyvelde, Bart
Daled, Simon
Deforce, Dieter
Dhaenens, Maarten
author_sort Verhelst, Sigrid
collection PubMed
description • There is a growing interest in epigenetic modifications, and more specifically in histone post-translational modifications (hPTMs). • We describe an MS-based workflow that is capable of tracking all possible hPTMs in an untargeted approach that makes use of human cells. • Improving the workflow and the user-friendliness in order to become a high throughput, easily applicable, toxicological screening assay is an ongoing effort.
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spelling pubmed-75089892020-09-28 Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity Verhelst, Sigrid De Clerck, Laura Willems, Sander Van Puyvelde, Bart Daled, Simon Deforce, Dieter Dhaenens, Maarten MethodsX Method Article • There is a growing interest in epigenetic modifications, and more specifically in histone post-translational modifications (hPTMs). • We describe an MS-based workflow that is capable of tracking all possible hPTMs in an untargeted approach that makes use of human cells. • Improving the workflow and the user-friendliness in order to become a high throughput, easily applicable, toxicological screening assay is an ongoing effort. Elsevier 2020-09-05 /pmc/articles/PMC7508989/ /pubmed/32995308 http://dx.doi.org/10.1016/j.mex.2020.101055 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Method Article
Verhelst, Sigrid
De Clerck, Laura
Willems, Sander
Van Puyvelde, Bart
Daled, Simon
Deforce, Dieter
Dhaenens, Maarten
Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title_full Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title_fullStr Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title_full_unstemmed Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title_short Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
title_sort comprehensive histone epigenetics: a mass spectrometry based screening assay to measure epigenetic toxicity
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7508989/
https://www.ncbi.nlm.nih.gov/pubmed/32995308
http://dx.doi.org/10.1016/j.mex.2020.101055
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