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Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses

Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyph...

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Autores principales: Yathisha, Neeragunda Shivaraj, Barbara, Plancot, Gügi, Bruno, Yogendra, Kambalagere, Jogaiah, Sudisha, Azeddine, Driouich, Sharatchandra, Ramasandra Govind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509185/
https://www.ncbi.nlm.nih.gov/pubmed/32995628
http://dx.doi.org/10.1016/j.heliyon.2020.e04948
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author Yathisha, Neeragunda Shivaraj
Barbara, Plancot
Gügi, Bruno
Yogendra, Kambalagere
Jogaiah, Sudisha
Azeddine, Driouich
Sharatchandra, Ramasandra Govind
author_facet Yathisha, Neeragunda Shivaraj
Barbara, Plancot
Gügi, Bruno
Yogendra, Kambalagere
Jogaiah, Sudisha
Azeddine, Driouich
Sharatchandra, Ramasandra Govind
author_sort Yathisha, Neeragunda Shivaraj
collection PubMed
description Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyphylla by evaluating its ability to recover the physiological, biochemical and morphological functions post desiccation. In order to understand the responses of Eragrostiella brachyphylla to desiccation and subsequent rehydration experiments were conducted in the hydrated state (HS), desiccated state (DS) and rehydrated state (RS). Scanning electron microscopy revealed significant changes between the three stages in the internal ultra-structures of leaves and stems. Compared to the other states, photosynthetic parameters such as chlorophyll a, chlorophyll b, total chlorophylland total carotenoid contents decreased significantly in the desiccated state. Superoxide radical (O2(•−)) content also increased, resulting in an oxidative burst during desiccation. Consequently, antioxidant enzymes such as catalase (CAT) superoxide dismutase (SOD) peroxidase (APX) and glutathione reductase (GR) activities were found to be significantly elevated in the desiccated state to avoid oxidative damage. Increased malondialdehyde (MDA) content and relative electrolyte leakage (REL) during desiccation provide evidence for membrane damage and loss of cell-wall integrity. During desiccation, the contents of osmolytes represented by sucrose and proline were found to increase to maintain cell structure integrity. After rehydration, all physiological, biochemical and morphological properties remain unchanged or slightly changed when compared to the hydrated state. Hence, we believe that these unique adaptations contribute to the remarkable desiccation-tolerance property of this plant.
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spelling pubmed-75091852020-09-28 Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses Yathisha, Neeragunda Shivaraj Barbara, Plancot Gügi, Bruno Yogendra, Kambalagere Jogaiah, Sudisha Azeddine, Driouich Sharatchandra, Ramasandra Govind Heliyon Research Article Eragrostiella brachyphylla is an angiosperm desiccation-tolerant resurrection plant, which can survive during desiccation in the air-dry state and recover completely on availability of water. The present study was conducted to understand the vegetative desiccation tolerance of Eragrostiella brachyphylla by evaluating its ability to recover the physiological, biochemical and morphological functions post desiccation. In order to understand the responses of Eragrostiella brachyphylla to desiccation and subsequent rehydration experiments were conducted in the hydrated state (HS), desiccated state (DS) and rehydrated state (RS). Scanning electron microscopy revealed significant changes between the three stages in the internal ultra-structures of leaves and stems. Compared to the other states, photosynthetic parameters such as chlorophyll a, chlorophyll b, total chlorophylland total carotenoid contents decreased significantly in the desiccated state. Superoxide radical (O2(•−)) content also increased, resulting in an oxidative burst during desiccation. Consequently, antioxidant enzymes such as catalase (CAT) superoxide dismutase (SOD) peroxidase (APX) and glutathione reductase (GR) activities were found to be significantly elevated in the desiccated state to avoid oxidative damage. Increased malondialdehyde (MDA) content and relative electrolyte leakage (REL) during desiccation provide evidence for membrane damage and loss of cell-wall integrity. During desiccation, the contents of osmolytes represented by sucrose and proline were found to increase to maintain cell structure integrity. After rehydration, all physiological, biochemical and morphological properties remain unchanged or slightly changed when compared to the hydrated state. Hence, we believe that these unique adaptations contribute to the remarkable desiccation-tolerance property of this plant. Elsevier 2020-09-18 /pmc/articles/PMC7509185/ /pubmed/32995628 http://dx.doi.org/10.1016/j.heliyon.2020.e04948 Text en © 2020 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Yathisha, Neeragunda Shivaraj
Barbara, Plancot
Gügi, Bruno
Yogendra, Kambalagere
Jogaiah, Sudisha
Azeddine, Driouich
Sharatchandra, Ramasandra Govind
Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title_full Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title_fullStr Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title_full_unstemmed Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title_short Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses
title_sort vegetative desiccation tolerance in eragrostiella brachyphylla: biochemical and physiological responses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509185/
https://www.ncbi.nlm.nih.gov/pubmed/32995628
http://dx.doi.org/10.1016/j.heliyon.2020.e04948
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