Cargando…
Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing
Litsea cubeba L. essential oil (LCEO) is a natural essential oil with considerable antimicrobial activity, and it can gradually replace some chemical additives in the food industry. However, the genetic evidences of stress response of bacteria under sub-lethal treatment with LCEO is limited. To this...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509438/ https://www.ncbi.nlm.nih.gov/pubmed/33013718 http://dx.doi.org/10.3389/fmicb.2020.01693 |
_version_ | 1783585596101361664 |
---|---|
author | Yang, Yunqiao Chen, Yunru Zhang, Geyin Sun, Junying Guo, Lei Jiang, Mingsheng Ou, Bingming Zhang, Weiyu Si, Hongbin |
author_facet | Yang, Yunqiao Chen, Yunru Zhang, Geyin Sun, Junying Guo, Lei Jiang, Mingsheng Ou, Bingming Zhang, Weiyu Si, Hongbin |
author_sort | Yang, Yunqiao |
collection | PubMed |
description | Litsea cubeba L. essential oil (LCEO) is a natural essential oil with considerable antimicrobial activity, and it can gradually replace some chemical additives in the food industry. However, the genetic evidences of stress response of bacteria under sub-lethal treatment with LCEO is limited. To this end, transcriptomic analysis of Staphylococcus aureus 29213 under a low concentration of LCEO was performed. Bacterial RNA samples were extracted from 1/4 MIC (0.07 μL/mL) of LCEO-treated and non-treated S. aureus 29213. The transcriptional results were obtained by RNA sequencing (RNA-Seq). After treated with LCEO of S. aureus 29213, 300, and 242 genes were significantly up and down-regulated. Up-regulated genes were mainly related to cell membrane (wall) stress stimulon including genes related to two-component regulatory system (VraS), histidine metabolism (hisABCD etc.) and L-lysine biosynthesis (thrA, lysC, asd etc.). Significant differences were also founded between LCEO-treated and non-treated groups in peptidoglycan biosynthesis related pathways. Down-regulated genes were related to nitrogen metabolism (NarGHIJ etc.), carotenoid biosynthesis (all) and pyruvate metabolism (phdA, pflB, pdhC etc.) of S. aureus 29213 in an LCEO-existing environment compared to the control. At the same time, we confirmed that LCEO can significantly affect the staphyloxanthin level of S. aureus 29213 for the first time, which is closely related to the redox state of S. aureus 29213. These evidences expanded the knowledge of stress response of S. aureus 29213 strain under sub-lethal concentration of LCEO. |
format | Online Article Text |
id | pubmed-7509438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75094382020-10-02 Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing Yang, Yunqiao Chen, Yunru Zhang, Geyin Sun, Junying Guo, Lei Jiang, Mingsheng Ou, Bingming Zhang, Weiyu Si, Hongbin Front Microbiol Microbiology Litsea cubeba L. essential oil (LCEO) is a natural essential oil with considerable antimicrobial activity, and it can gradually replace some chemical additives in the food industry. However, the genetic evidences of stress response of bacteria under sub-lethal treatment with LCEO is limited. To this end, transcriptomic analysis of Staphylococcus aureus 29213 under a low concentration of LCEO was performed. Bacterial RNA samples were extracted from 1/4 MIC (0.07 μL/mL) of LCEO-treated and non-treated S. aureus 29213. The transcriptional results were obtained by RNA sequencing (RNA-Seq). After treated with LCEO of S. aureus 29213, 300, and 242 genes were significantly up and down-regulated. Up-regulated genes were mainly related to cell membrane (wall) stress stimulon including genes related to two-component regulatory system (VraS), histidine metabolism (hisABCD etc.) and L-lysine biosynthesis (thrA, lysC, asd etc.). Significant differences were also founded between LCEO-treated and non-treated groups in peptidoglycan biosynthesis related pathways. Down-regulated genes were related to nitrogen metabolism (NarGHIJ etc.), carotenoid biosynthesis (all) and pyruvate metabolism (phdA, pflB, pdhC etc.) of S. aureus 29213 in an LCEO-existing environment compared to the control. At the same time, we confirmed that LCEO can significantly affect the staphyloxanthin level of S. aureus 29213 for the first time, which is closely related to the redox state of S. aureus 29213. These evidences expanded the knowledge of stress response of S. aureus 29213 strain under sub-lethal concentration of LCEO. Frontiers Media S.A. 2020-09-08 /pmc/articles/PMC7509438/ /pubmed/33013718 http://dx.doi.org/10.3389/fmicb.2020.01693 Text en Copyright © 2020 Yang, Chen, Zhang, Sun, Guo, Jiang, Ou, Zhang and Si. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yang, Yunqiao Chen, Yunru Zhang, Geyin Sun, Junying Guo, Lei Jiang, Mingsheng Ou, Bingming Zhang, Weiyu Si, Hongbin Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title | Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title_full | Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title_fullStr | Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title_full_unstemmed | Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title_short | Transcriptomic Analysis of Staphylococcus aureus Under the Stress Condition Caused by Litsea cubeba L. Essential Oil via RNA Sequencing |
title_sort | transcriptomic analysis of staphylococcus aureus under the stress condition caused by litsea cubeba l. essential oil via rna sequencing |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509438/ https://www.ncbi.nlm.nih.gov/pubmed/33013718 http://dx.doi.org/10.3389/fmicb.2020.01693 |
work_keys_str_mv | AT yangyunqiao transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT chenyunru transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT zhanggeyin transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT sunjunying transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT guolei transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT jiangmingsheng transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT oubingming transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT zhangweiyu transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing AT sihongbin transcriptomicanalysisofstaphylococcusaureusunderthestressconditioncausedbylitseacubebalessentialoilviarnasequencing |