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Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509470/ https://www.ncbi.nlm.nih.gov/pubmed/33005784 http://dx.doi.org/10.1016/j.heliyon.2020.e04941 |
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author | Feodorova, Yana Tomova, Teodora Minchev, Danail Turiyski, Valentin Draganov, Marian Argirova, Mariana |
author_facet | Feodorova, Yana Tomova, Teodora Minchev, Danail Turiyski, Valentin Draganov, Marian Argirova, Mariana |
author_sort | Feodorova, Yana |
collection | PubMed |
description | While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used 70%-methanol Ginkgo biloba kernel extract, quantified its bioactive constituents and tested their cytotoxic effect on two cancer cell lines, A2058 and HCT116, and the non-tumor cell line McCoy-Plovdiv. We studied the biological effect of the extract by real-time analysis in the xCELLigence system, WST-1 assay and LIVE/DEAD viability assay. We show that the extract significantly perturbed the viability of cancer cells in a concentration- and time-dependent manner. In contrast, non-cancerous McCoy-Plovdiv cells sustained their proliferation potential even at high concentrations of the extract. Therefore, we propose that the active constituents of the Ginkgo biloba endosperm extract may interact additively or synergistically to protect against cancer. |
format | Online Article Text |
id | pubmed-7509470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75094702020-09-30 Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines Feodorova, Yana Tomova, Teodora Minchev, Danail Turiyski, Valentin Draganov, Marian Argirova, Mariana Heliyon Research Article While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used 70%-methanol Ginkgo biloba kernel extract, quantified its bioactive constituents and tested their cytotoxic effect on two cancer cell lines, A2058 and HCT116, and the non-tumor cell line McCoy-Plovdiv. We studied the biological effect of the extract by real-time analysis in the xCELLigence system, WST-1 assay and LIVE/DEAD viability assay. We show that the extract significantly perturbed the viability of cancer cells in a concentration- and time-dependent manner. In contrast, non-cancerous McCoy-Plovdiv cells sustained their proliferation potential even at high concentrations of the extract. Therefore, we propose that the active constituents of the Ginkgo biloba endosperm extract may interact additively or synergistically to protect against cancer. Elsevier 2020-09-19 /pmc/articles/PMC7509470/ /pubmed/33005784 http://dx.doi.org/10.1016/j.heliyon.2020.e04941 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Feodorova, Yana Tomova, Teodora Minchev, Danail Turiyski, Valentin Draganov, Marian Argirova, Mariana Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title | Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title_full | Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title_fullStr | Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title_full_unstemmed | Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title_short | Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines |
title_sort | cytotoxic effect of ginkgo biloba kernel extract on hct116 and a2058 cancer cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509470/ https://www.ncbi.nlm.nih.gov/pubmed/33005784 http://dx.doi.org/10.1016/j.heliyon.2020.e04941 |
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