Cargando…

Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines

While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used...

Descripción completa

Detalles Bibliográficos
Autores principales: Feodorova, Yana, Tomova, Teodora, Minchev, Danail, Turiyski, Valentin, Draganov, Marian, Argirova, Mariana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509470/
https://www.ncbi.nlm.nih.gov/pubmed/33005784
http://dx.doi.org/10.1016/j.heliyon.2020.e04941
_version_ 1783585603092217856
author Feodorova, Yana
Tomova, Teodora
Minchev, Danail
Turiyski, Valentin
Draganov, Marian
Argirova, Mariana
author_facet Feodorova, Yana
Tomova, Teodora
Minchev, Danail
Turiyski, Valentin
Draganov, Marian
Argirova, Mariana
author_sort Feodorova, Yana
collection PubMed
description While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used 70%-methanol Ginkgo biloba kernel extract, quantified its bioactive constituents and tested their cytotoxic effect on two cancer cell lines, A2058 and HCT116, and the non-tumor cell line McCoy-Plovdiv. We studied the biological effect of the extract by real-time analysis in the xCELLigence system, WST-1 assay and LIVE/DEAD viability assay. We show that the extract significantly perturbed the viability of cancer cells in a concentration- and time-dependent manner. In contrast, non-cancerous McCoy-Plovdiv cells sustained their proliferation potential even at high concentrations of the extract. Therefore, we propose that the active constituents of the Ginkgo biloba endosperm extract may interact additively or synergistically to protect against cancer.
format Online
Article
Text
id pubmed-7509470
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75094702020-09-30 Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines Feodorova, Yana Tomova, Teodora Minchev, Danail Turiyski, Valentin Draganov, Marian Argirova, Mariana Heliyon Research Article While the pharmacology of Ginkgo biloba leaf extract has been studied extensively, little is known about the pharmacological potential of Ginkgo biloba seeds, although they contain similar active ingredients that are responsible for the therapeutic effects of the leaf extract. In this study we used 70%-methanol Ginkgo biloba kernel extract, quantified its bioactive constituents and tested their cytotoxic effect on two cancer cell lines, A2058 and HCT116, and the non-tumor cell line McCoy-Plovdiv. We studied the biological effect of the extract by real-time analysis in the xCELLigence system, WST-1 assay and LIVE/DEAD viability assay. We show that the extract significantly perturbed the viability of cancer cells in a concentration- and time-dependent manner. In contrast, non-cancerous McCoy-Plovdiv cells sustained their proliferation potential even at high concentrations of the extract. Therefore, we propose that the active constituents of the Ginkgo biloba endosperm extract may interact additively or synergistically to protect against cancer. Elsevier 2020-09-19 /pmc/articles/PMC7509470/ /pubmed/33005784 http://dx.doi.org/10.1016/j.heliyon.2020.e04941 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Feodorova, Yana
Tomova, Teodora
Minchev, Danail
Turiyski, Valentin
Draganov, Marian
Argirova, Mariana
Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title_full Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title_fullStr Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title_full_unstemmed Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title_short Cytotoxic effect of Ginkgo biloba kernel extract on HCT116 and A2058 cancer cell lines
title_sort cytotoxic effect of ginkgo biloba kernel extract on hct116 and a2058 cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509470/
https://www.ncbi.nlm.nih.gov/pubmed/33005784
http://dx.doi.org/10.1016/j.heliyon.2020.e04941
work_keys_str_mv AT feodorovayana cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines
AT tomovateodora cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines
AT minchevdanail cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines
AT turiyskivalentin cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines
AT draganovmarian cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines
AT argirovamariana cytotoxiceffectofginkgobilobakernelextractonhct116anda2058cancercelllines