Cargando…
TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells
Gastrointestinal stromal tumors (GISTs) are the most common pathologic type of mesenchymal tumor in the digestive tract. Patients with GIST face the risk of metastasis, postoperative recurrence and imatinib mesylate (IM) resistance. Mitochondrial Tu translation elongation factor (TUFM) is highly exp...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509754/ https://www.ncbi.nlm.nih.gov/pubmed/32994813 http://dx.doi.org/10.3892/ol.2020.12113 |
_version_ | 1783585662683840512 |
---|---|
author | Weng, Xiaoyuan Zheng, Song Shui, Hanli Lin, Guosheng Zhou, Yongjian |
author_facet | Weng, Xiaoyuan Zheng, Song Shui, Hanli Lin, Guosheng Zhou, Yongjian |
author_sort | Weng, Xiaoyuan |
collection | PubMed |
description | Gastrointestinal stromal tumors (GISTs) are the most common pathologic type of mesenchymal tumor in the digestive tract. Patients with GIST face the risk of metastasis, postoperative recurrence and imatinib mesylate (IM) resistance. Mitochondrial Tu translation elongation factor (TUFM) is highly expressed in GISTs, and is associated with oncogenesis, progression and prognosis. There is evidence that TUFM is involved in tumor invasion and metastasis. However, the effect of TUFM on GIST-T1 cells and the IM-resistant GIST-IR cell line remains unclear. The present study aimed to evaluate the effects of TUFM on the proliferation, migration and apoptosis of GIST cells in vitro. TUFM short hairpin (sh)RNA expression plasmids were transfected into GIST-T1 and GIST-IR cells by electroporation. The expression levels of enhanced green fluorescent protein were observed by fluorescence microscopy to evaluate the electroporation efficiency. The expression levels of TUFM were detected by western blot analysis and reverse transcription-quantitative PCR. Cell proliferation was assessed by counting cells and using a Cell Counting Kit-8 assay. Cell migration was analyzed using wound healing and Transwell migration assays. Cell cycle distribution and late apoptosis were assessed by flow cytometry. TUFM shRNA expression plasmids were successfully transfected into the GIST cell line by electroporation. The transfection efficiency was >75%, and the TUFM gene silencing efficiency was 73.2±1.4%. TUFM-knockdown decreased the proliferation and migration capacity of GIST-T1 and GIST-IR cells. The proportion of cells in the pre-G1 stage was increased without change in the proportions of cells in the G(1), S and G(2)/M stages after TUFM silencing in GIST-T1 and GIST-IR cells. TUFM may be related to GIST infiltration and metastatic recurrence, suggesting that TUFM may be an effective target for preventing the progression and metastasis of GISTs. |
format | Online Article Text |
id | pubmed-7509754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-75097542020-09-28 TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells Weng, Xiaoyuan Zheng, Song Shui, Hanli Lin, Guosheng Zhou, Yongjian Oncol Lett Articles Gastrointestinal stromal tumors (GISTs) are the most common pathologic type of mesenchymal tumor in the digestive tract. Patients with GIST face the risk of metastasis, postoperative recurrence and imatinib mesylate (IM) resistance. Mitochondrial Tu translation elongation factor (TUFM) is highly expressed in GISTs, and is associated with oncogenesis, progression and prognosis. There is evidence that TUFM is involved in tumor invasion and metastasis. However, the effect of TUFM on GIST-T1 cells and the IM-resistant GIST-IR cell line remains unclear. The present study aimed to evaluate the effects of TUFM on the proliferation, migration and apoptosis of GIST cells in vitro. TUFM short hairpin (sh)RNA expression plasmids were transfected into GIST-T1 and GIST-IR cells by electroporation. The expression levels of enhanced green fluorescent protein were observed by fluorescence microscopy to evaluate the electroporation efficiency. The expression levels of TUFM were detected by western blot analysis and reverse transcription-quantitative PCR. Cell proliferation was assessed by counting cells and using a Cell Counting Kit-8 assay. Cell migration was analyzed using wound healing and Transwell migration assays. Cell cycle distribution and late apoptosis were assessed by flow cytometry. TUFM shRNA expression plasmids were successfully transfected into the GIST cell line by electroporation. The transfection efficiency was >75%, and the TUFM gene silencing efficiency was 73.2±1.4%. TUFM-knockdown decreased the proliferation and migration capacity of GIST-T1 and GIST-IR cells. The proportion of cells in the pre-G1 stage was increased without change in the proportions of cells in the G(1), S and G(2)/M stages after TUFM silencing in GIST-T1 and GIST-IR cells. TUFM may be related to GIST infiltration and metastatic recurrence, suggesting that TUFM may be an effective target for preventing the progression and metastasis of GISTs. D.A. Spandidos 2020-11 2020-09-17 /pmc/articles/PMC7509754/ /pubmed/32994813 http://dx.doi.org/10.3892/ol.2020.12113 Text en Copyright: © Weng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Weng, Xiaoyuan Zheng, Song Shui, Hanli Lin, Guosheng Zhou, Yongjian TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title | TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title_full | TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title_fullStr | TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title_full_unstemmed | TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title_short | TUFM-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
title_sort | tufm-knockdown inhibits the migration and proliferation of gastrointestinal stromal tumor cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509754/ https://www.ncbi.nlm.nih.gov/pubmed/32994813 http://dx.doi.org/10.3892/ol.2020.12113 |
work_keys_str_mv | AT wengxiaoyuan tufmknockdowninhibitsthemigrationandproliferationofgastrointestinalstromaltumorcells AT zhengsong tufmknockdowninhibitsthemigrationandproliferationofgastrointestinalstromaltumorcells AT shuihanli tufmknockdowninhibitsthemigrationandproliferationofgastrointestinalstromaltumorcells AT linguosheng tufmknockdowninhibitsthemigrationandproliferationofgastrointestinalstromaltumorcells AT zhouyongjian tufmknockdowninhibitsthemigrationandproliferationofgastrointestinalstromaltumorcells |