Cargando…

Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis

Platelet rich plasma contains a collection of growth factors, and an optimal formulation, named O-rPRP, contains the highest possible concentration of growth factors. PURPOSE: Challenging the healing power of O-rPRP in a high-galactose diet-induced premature ovarian insufficiency (POI) experimental...

Descripción completa

Detalles Bibliográficos
Autores principales: El Bakly, Wesam, Medhat, Marwa, Shafei, Mohamed, Tash, Reham, Elrefai, Mohamed, Shoukry, Yousef, Omar, Nesreen Nabil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509792/
https://www.ncbi.nlm.nih.gov/pubmed/33005806
http://dx.doi.org/10.1016/j.heliyon.2020.e05006
_version_ 1783585670561792000
author El Bakly, Wesam
Medhat, Marwa
Shafei, Mohamed
Tash, Reham
Elrefai, Mohamed
Shoukry, Yousef
Omar, Nesreen Nabil
author_facet El Bakly, Wesam
Medhat, Marwa
Shafei, Mohamed
Tash, Reham
Elrefai, Mohamed
Shoukry, Yousef
Omar, Nesreen Nabil
author_sort El Bakly, Wesam
collection PubMed
description Platelet rich plasma contains a collection of growth factors, and an optimal formulation, named O-rPRP, contains the highest possible concentration of growth factors. PURPOSE: Challenging the healing power of O-rPRP in a high-galactose diet-induced premature ovarian insufficiency (POI) experimental rat model. METHODS: Rats were divided into four groups of ten rats each and treated for four week as follows; 1) the control group, fed with normal diet and received intraperitoneal (i.p.) injection of PBS once/week; 2) the POI group, fed with galactose diet (50%) and received PBS (i.p.) once/week; 3) the POI/O-rPRP group, fed a 50% galactose diet and received O-rPRP (i.p.) once/week; 4) the O-rPRP group (negative control), fed with a normal diet and received O-rPRP (i.p.) once/week. The levels of galactose, follicle stimulating hormone, 17 β-estradiol, anti-mullerian hormone and inhibin B were measured in serum samples. Western blotting and quantitative real-time PCR assays were employed to investigate the levels of miR-223, β1 integrin, p70S6k and MCL-1 in ovarian tissues. RESULTS: After O-rPRP treatment, β1 integrin expression was enhanced, and miR-223 expression was decreased. Unlike the untreated galactose group, in the group treated with O-rPRP, p70S6k and MCL-1 expression levels were increased, indicating that the mTOR growth signaling pathway was active and that apoptosis was inactive. After the introduction of O-rPRP, the number of follicles and the follicular maturation improved, which was consistent with the improvement of inhibin B levels and subsequent inhibition of FSH. CONCLUSION: O-rPRP inhibited galactose-induced excessive atresia and provided an overall protective effect on the ovarian follicles.
format Online
Article
Text
id pubmed-7509792
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75097922020-09-30 Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis El Bakly, Wesam Medhat, Marwa Shafei, Mohamed Tash, Reham Elrefai, Mohamed Shoukry, Yousef Omar, Nesreen Nabil Heliyon Research Article Platelet rich plasma contains a collection of growth factors, and an optimal formulation, named O-rPRP, contains the highest possible concentration of growth factors. PURPOSE: Challenging the healing power of O-rPRP in a high-galactose diet-induced premature ovarian insufficiency (POI) experimental rat model. METHODS: Rats were divided into four groups of ten rats each and treated for four week as follows; 1) the control group, fed with normal diet and received intraperitoneal (i.p.) injection of PBS once/week; 2) the POI group, fed with galactose diet (50%) and received PBS (i.p.) once/week; 3) the POI/O-rPRP group, fed a 50% galactose diet and received O-rPRP (i.p.) once/week; 4) the O-rPRP group (negative control), fed with a normal diet and received O-rPRP (i.p.) once/week. The levels of galactose, follicle stimulating hormone, 17 β-estradiol, anti-mullerian hormone and inhibin B were measured in serum samples. Western blotting and quantitative real-time PCR assays were employed to investigate the levels of miR-223, β1 integrin, p70S6k and MCL-1 in ovarian tissues. RESULTS: After O-rPRP treatment, β1 integrin expression was enhanced, and miR-223 expression was decreased. Unlike the untreated galactose group, in the group treated with O-rPRP, p70S6k and MCL-1 expression levels were increased, indicating that the mTOR growth signaling pathway was active and that apoptosis was inactive. After the introduction of O-rPRP, the number of follicles and the follicular maturation improved, which was consistent with the improvement of inhibin B levels and subsequent inhibition of FSH. CONCLUSION: O-rPRP inhibited galactose-induced excessive atresia and provided an overall protective effect on the ovarian follicles. Elsevier 2020-09-21 /pmc/articles/PMC7509792/ /pubmed/33005806 http://dx.doi.org/10.1016/j.heliyon.2020.e05006 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
El Bakly, Wesam
Medhat, Marwa
Shafei, Mohamed
Tash, Reham
Elrefai, Mohamed
Shoukry, Yousef
Omar, Nesreen Nabil
Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title_full Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title_fullStr Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title_full_unstemmed Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title_short Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
title_sort optimized platelet rich plasma releasate (o-rprp) repairs galactosemia-induced ovarian follicular loss in rats by activating mtor signaling and inhibiting apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509792/
https://www.ncbi.nlm.nih.gov/pubmed/33005806
http://dx.doi.org/10.1016/j.heliyon.2020.e05006
work_keys_str_mv AT elbaklywesam optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT medhatmarwa optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT shafeimohamed optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT tashreham optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT elrefaimohamed optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT shoukryyousef optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis
AT omarnesreennabil optimizedplateletrichplasmareleasateorprprepairsgalactosemiainducedovarianfollicularlossinratsbyactivatingmtorsignalingandinhibitingapoptosis