Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

[Image: see text] Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report...

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Autores principales: Murray, Kathryn A., Tomás, Ruben M. F., Gibson, Matthew I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509910/
https://www.ncbi.nlm.nih.gov/pubmed/32984779
http://dx.doi.org/10.1021/acsabm.0c00638
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author Murray, Kathryn A.
Tomás, Ruben M. F.
Gibson, Matthew I.
author_facet Murray, Kathryn A.
Tomás, Ruben M. F.
Gibson, Matthew I.
author_sort Murray, Kathryn A.
collection PubMed
description [Image: see text] Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
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spelling pubmed-75099102020-09-24 Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants Murray, Kathryn A. Tomás, Ruben M. F. Gibson, Matthew I. ACS Appl Bio Mater [Image: see text] Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies. American Chemical Society 2020-08-18 2020-09-21 /pmc/articles/PMC7509910/ /pubmed/32984779 http://dx.doi.org/10.1021/acsabm.0c00638 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Murray, Kathryn A.
Tomás, Ruben M. F.
Gibson, Matthew I.
Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title_full Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title_fullStr Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title_full_unstemmed Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title_short Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants
title_sort low dmso cryopreservation of stem cells enabled by macromolecular cryoprotectants
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7509910/
https://www.ncbi.nlm.nih.gov/pubmed/32984779
http://dx.doi.org/10.1021/acsabm.0c00638
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