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Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum

BACKGROUND: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulatio...

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Autores principales: Jiang, Heng, Zhang, Nan, Ji, Caihong, Meng, Xiangkun, Qian, Kun, Zheng, Yang, Wang, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510082/
https://www.ncbi.nlm.nih.gov/pubmed/32967608
http://dx.doi.org/10.1186/s12864-020-07070-3
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author Jiang, Heng
Zhang, Nan
Ji, Caihong
Meng, Xiangkun
Qian, Kun
Zheng, Yang
Wang, Jianjun
author_facet Jiang, Heng
Zhang, Nan
Ji, Caihong
Meng, Xiangkun
Qian, Kun
Zheng, Yang
Wang, Jianjun
author_sort Jiang, Heng
collection PubMed
description BACKGROUND: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing. RESULTS: RNAi-mediated suppression of TcAMPKα increased whole-body triglyceride (TG) level and the ratio between glucose and trehalose, as was confirmed by in vivo treatment with the AMPK-activating compound, 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR). A total of 1184 differentially expressed genes (DEGs) were identified between dsTcAMPKα-injected and dsEGFP-injected beetles. These include genes involved in lipid and carbohydrate metabolism as well as insulin/insulin-like growth factor signaling (IIS). Real-time quantitative polymerase chain reaction analysis confirmed the differential expression of selected genes. Interestingly, metabolism-related transcription factors such as sterol regulatory element-binding protein 1 (SREBP1) and carbohydrate response element-binding protein (ChREBP) were also significantly upregulated in dsTcAMPKα-injected beetles. CONCLUSIONS: AMPK plays a critical role in the regulation of beetle metabolism. The findings of DEGs involved in lipid and carbohydrate metabolism provide valuable insight into the role of AMPK signaling in the transcriptional regulation of insect metabolism.
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spelling pubmed-75100822020-09-24 Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum Jiang, Heng Zhang, Nan Ji, Caihong Meng, Xiangkun Qian, Kun Zheng, Yang Wang, Jianjun BMC Genomics Research Article BACKGROUND: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing. RESULTS: RNAi-mediated suppression of TcAMPKα increased whole-body triglyceride (TG) level and the ratio between glucose and trehalose, as was confirmed by in vivo treatment with the AMPK-activating compound, 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR). A total of 1184 differentially expressed genes (DEGs) were identified between dsTcAMPKα-injected and dsEGFP-injected beetles. These include genes involved in lipid and carbohydrate metabolism as well as insulin/insulin-like growth factor signaling (IIS). Real-time quantitative polymerase chain reaction analysis confirmed the differential expression of selected genes. Interestingly, metabolism-related transcription factors such as sterol regulatory element-binding protein 1 (SREBP1) and carbohydrate response element-binding protein (ChREBP) were also significantly upregulated in dsTcAMPKα-injected beetles. CONCLUSIONS: AMPK plays a critical role in the regulation of beetle metabolism. The findings of DEGs involved in lipid and carbohydrate metabolism provide valuable insight into the role of AMPK signaling in the transcriptional regulation of insect metabolism. BioMed Central 2020-09-23 /pmc/articles/PMC7510082/ /pubmed/32967608 http://dx.doi.org/10.1186/s12864-020-07070-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Jiang, Heng
Zhang, Nan
Ji, Caihong
Meng, Xiangkun
Qian, Kun
Zheng, Yang
Wang, Jianjun
Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title_full Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title_fullStr Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title_full_unstemmed Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title_short Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum
title_sort metabolic and transcriptome responses of rnai-mediated ampkα knockdown in tribolium castaneum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510082/
https://www.ncbi.nlm.nih.gov/pubmed/32967608
http://dx.doi.org/10.1186/s12864-020-07070-3
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