The complete mitochondrial genome of Hemerobius spodipennis (Neuroptera: Hemerobiidae)

The complete mitochondrial genome of Hemerobius spodipennis Yang, 1987 was sequenced in this study. The complete mitochondrial genome is a typical double-stranded circular molecule of 16,343 bp (GenBank accession number: MT268963) comprising of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. The gene order is identical to that of the putative ancestral arrangement of insects and other lacewings. All protein-coding genes initiate with ATN, except COI use CGA as start codons and terminate with TAG or TAA, expect ND5 and ND4 use TA– or a single T–– residue as the stop codon. All tRNAs, ranging from 63 to 72 bp, can be folded into typical clover-leaf secondary structure except for tRNA(Ser(AGN)), in which the dihydrouridine (DHU) arm did not form a stable stem-loop structure. The control region is 1433 bp long with an A + T content of 91.4%. In the sampled families of Neuroptera, each family showed a monophyletic cluster and Polystoechotidae + Rapismatidae, Hemerobiidae + (Chrysopidae + (Polystoechotidae + Rapismatidae)), are recovered in phylogenetic analyses with high supports.