Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Whole-genome sequencing had greater ab...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekizuka, Tsuyoshi, Katsukawa, Chihiro, Kuroda, Makoto, Shibayama, Keigo, Otsuji, Ken, Saito, Mitsumasa, Yamamoto, Akihiko, Iwaki, Masaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510715/
https://www.ncbi.nlm.nih.gov/pubmed/32946740
http://dx.doi.org/10.3201/eid2610.200086
_version_ 1783585848752603136
author Sekizuka, Tsuyoshi
Katsukawa, Chihiro
Kuroda, Makoto
Shibayama, Keigo
Otsuji, Ken
Saito, Mitsumasa
Yamamoto, Akihiko
Iwaki, Masaaki
author_facet Sekizuka, Tsuyoshi
Katsukawa, Chihiro
Kuroda, Makoto
Shibayama, Keigo
Otsuji, Ken
Saito, Mitsumasa
Yamamoto, Akihiko
Iwaki, Masaaki
author_sort Sekizuka, Tsuyoshi
collection PubMed
description We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Whole-genome sequencing had greater ability to discriminate lineages at high resolution.
format Online
Article
Text
id pubmed-7510715
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-75107152020-10-02 Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans Sekizuka, Tsuyoshi Katsukawa, Chihiro Kuroda, Makoto Shibayama, Keigo Otsuji, Ken Saito, Mitsumasa Yamamoto, Akihiko Iwaki, Masaaki Emerg Infect Dis Dispatch We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Whole-genome sequencing had greater ability to discriminate lineages at high resolution. Centers for Disease Control and Prevention 2020-10 /pmc/articles/PMC7510715/ /pubmed/32946740 http://dx.doi.org/10.3201/eid2610.200086 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Sekizuka, Tsuyoshi
Katsukawa, Chihiro
Kuroda, Makoto
Shibayama, Keigo
Otsuji, Ken
Saito, Mitsumasa
Yamamoto, Akihiko
Iwaki, Masaaki
Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title_full Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title_fullStr Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title_full_unstemmed Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title_short Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans
title_sort limitations of ribotyping as genotyping method for corynebacterium ulcerans
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510715/
https://www.ncbi.nlm.nih.gov/pubmed/32946740
http://dx.doi.org/10.3201/eid2610.200086
work_keys_str_mv AT sekizukatsuyoshi limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT katsukawachihiro limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT kurodamakoto limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT shibayamakeigo limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT otsujiken limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT saitomitsumasa limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT yamamotoakihiko limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans
AT iwakimasaaki limitationsofribotypingasgenotypingmethodforcorynebacteriumulcerans

Ejemplares similares