Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR

To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivit...

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Detalles Bibliográficos
Autores principales: Kim, So Yeon, Lee, Jaehyeon, Sung, Heungsup, Lee, Hyukmin, Han, Myung Guk, Yoo, Cheon Kwon, Lee, Sang Won, Hong, Ki Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510748/
https://www.ncbi.nlm.nih.gov/pubmed/32844739
http://dx.doi.org/10.3201/eid2610.201955
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author Kim, So Yeon
Lee, Jaehyeon
Sung, Heungsup
Lee, Hyukmin
Han, Myung Guk
Yoo, Cheon Kwon
Lee, Sang Won
Hong, Ki Ho
author_facet Kim, So Yeon
Lee, Jaehyeon
Sung, Heungsup
Lee, Hyukmin
Han, Myung Guk
Yoo, Cheon Kwon
Lee, Sang Won
Hong, Ki Ho
author_sort Kim, So Yeon
collection PubMed
description To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.
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spelling pubmed-75107482020-10-02 Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR Kim, So Yeon Lee, Jaehyeon Sung, Heungsup Lee, Hyukmin Han, Myung Guk Yoo, Cheon Kwon Lee, Sang Won Hong, Ki Ho Emerg Infect Dis Dispatch To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method. Centers for Disease Control and Prevention 2020-10 /pmc/articles/PMC7510748/ /pubmed/32844739 http://dx.doi.org/10.3201/eid2610.201955 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Kim, So Yeon
Lee, Jaehyeon
Sung, Heungsup
Lee, Hyukmin
Han, Myung Guk
Yoo, Cheon Kwon
Lee, Sang Won
Hong, Ki Ho
Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title_full Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title_fullStr Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title_full_unstemmed Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title_short Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR
title_sort pooling upper respiratory specimens for rapid mass screening of covid-19 by real-time rt-pcr
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510748/
https://www.ncbi.nlm.nih.gov/pubmed/32844739
http://dx.doi.org/10.3201/eid2610.201955
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