Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet

There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the E...

Descripción completa

Detalles Bibliográficos
Autores principales: Krüttgen, Alexander, Cornelissen, Christian G., Dreher, Michael, Hornef, Mathias W., Imöhl, Matthias, Kleines, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510775/
https://www.ncbi.nlm.nih.gov/pubmed/32979407
http://dx.doi.org/10.1016/j.jviromet.2020.113978
_version_ 1783585862337953792
author Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
author_facet Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
author_sort Krüttgen, Alexander
collection PubMed
description There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the Elecsys Anti-SARS-CoV-2 assay (Roche), and the ID Screen SARS-CoV-2-N IgG indirect kit (IDVet). We determined a sensitivity of 95.5 %, 95.5 %, and 100 % and a specificity of 90.5 %, 96.2 %, and 92.5 % for the DiaSorin assay, the Roche assay, and the IDVet assay, respectively. We conclude that serologic assays combining very high sensitivity and specificity are still not commercially available for SARS-CoV-2. For maximizing sensitivity and specificity of SARS-CoV-2 serological diagnostics, the combination of two assays may be helpful.
format Online
Article
Text
id pubmed-7510775
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-75107752020-09-24 Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet Krüttgen, Alexander Cornelissen, Christian G. Dreher, Michael Hornef, Mathias W. Imöhl, Matthias Kleines, Michael J Virol Methods Article There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the Elecsys Anti-SARS-CoV-2 assay (Roche), and the ID Screen SARS-CoV-2-N IgG indirect kit (IDVet). We determined a sensitivity of 95.5 %, 95.5 %, and 100 % and a specificity of 90.5 %, 96.2 %, and 92.5 % for the DiaSorin assay, the Roche assay, and the IDVet assay, respectively. We conclude that serologic assays combining very high sensitivity and specificity are still not commercially available for SARS-CoV-2. For maximizing sensitivity and specificity of SARS-CoV-2 serological diagnostics, the combination of two assays may be helpful. Elsevier B.V. 2021-01 2020-09-23 /pmc/articles/PMC7510775/ /pubmed/32979407 http://dx.doi.org/10.1016/j.jviromet.2020.113978 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Krüttgen, Alexander
Cornelissen, Christian G.
Dreher, Michael
Hornef, Mathias W.
Imöhl, Matthias
Kleines, Michael
Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title_full Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title_fullStr Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title_full_unstemmed Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title_short Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
title_sort determination of sars-cov-2 antibodies with assays from diasorin, roche and idvet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510775/
https://www.ncbi.nlm.nih.gov/pubmed/32979407
http://dx.doi.org/10.1016/j.jviromet.2020.113978
work_keys_str_mv AT kruttgenalexander determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet
AT cornelissenchristiang determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet
AT drehermichael determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet
AT hornefmathiasw determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet
AT imohlmatthias determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet
AT kleinesmichael determinationofsarscov2antibodieswithassaysfromdiasorinrocheandidvet

Ejemplares similares