Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation

The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus prec...

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Autores principales: Alexander, Marina R., Rootes, Christina L., van Vuren, Petrus Jansen, Stewart, Cameron R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510776/
https://www.ncbi.nlm.nih.gov/pubmed/32979405
http://dx.doi.org/10.1016/j.jviromet.2020.113977
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author Alexander, Marina R.
Rootes, Christina L.
van Vuren, Petrus Jansen
Stewart, Cameron R.
author_facet Alexander, Marina R.
Rootes, Christina L.
van Vuren, Petrus Jansen
Stewart, Cameron R.
author_sort Alexander, Marina R.
collection PubMed
description The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a ∼1.5 log(10) increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas.
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spelling pubmed-75107762020-09-24 Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation Alexander, Marina R. Rootes, Christina L. van Vuren, Petrus Jansen Stewart, Cameron R. J Virol Methods Short Communication The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a ∼1.5 log(10) increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas. Published by Elsevier B.V. 2020-12 2020-09-23 /pmc/articles/PMC7510776/ /pubmed/32979405 http://dx.doi.org/10.1016/j.jviromet.2020.113977 Text en Crown Copyright © 2020 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Alexander, Marina R.
Rootes, Christina L.
van Vuren, Petrus Jansen
Stewart, Cameron R.
Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title_full Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title_fullStr Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title_full_unstemmed Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title_short Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation
title_sort concentration of infectious sars-cov-2 by polyethylene glycol precipitation
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510776/
https://www.ncbi.nlm.nih.gov/pubmed/32979405
http://dx.doi.org/10.1016/j.jviromet.2020.113977
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