Cargando…

Molecular classification of the placebo effect in nausea

In this proof-of-concept study, we tested whether placebo effects can be monitored and predicted by plasma proteins. In a randomized controlled design, 90 participants were exposed to a nauseating stimulus on two separate days and were randomly allocated to placebo treatment or no treatment on the s...

Descripción completa

Detalles Bibliográficos
Autores principales: Meissner, Karin, Lutter, Dominik, von Toerne, Christine, Haile, Anja, Woods, Stephen C., Hoffmann, Verena, Ohmayer, Uli, Hauck, Stefanie M., Tschoep, Matthias H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511022/
https://www.ncbi.nlm.nih.gov/pubmed/32966280
http://dx.doi.org/10.1371/journal.pone.0238533
_version_ 1783585896712372224
author Meissner, Karin
Lutter, Dominik
von Toerne, Christine
Haile, Anja
Woods, Stephen C.
Hoffmann, Verena
Ohmayer, Uli
Hauck, Stefanie M.
Tschoep, Matthias H.
author_facet Meissner, Karin
Lutter, Dominik
von Toerne, Christine
Haile, Anja
Woods, Stephen C.
Hoffmann, Verena
Ohmayer, Uli
Hauck, Stefanie M.
Tschoep, Matthias H.
author_sort Meissner, Karin
collection PubMed
description In this proof-of-concept study, we tested whether placebo effects can be monitored and predicted by plasma proteins. In a randomized controlled design, 90 participants were exposed to a nauseating stimulus on two separate days and were randomly allocated to placebo treatment or no treatment on the second day. Significant placebo effects on nausea, motion sickness, and (in females) gastric activity could be verified. Using label-free tandem mass spectrometry, 74 differentially regulated proteins were identified as correlates of the placebo effect. Gene ontology (GO) enrichment analyses identified acute-phase proteins and microinflammatory proteins to be involved, and the identified GO signatures predicted day-adjusted scores of nausea indices in the placebo group. We also performed GO enrichment analyses of specific plasma proteins predictable by the experimental factors or their interactions and identified ‘grooming behavior’ as a prominent hit. Finally, Receiver Operator Characteristics (ROC) allowed to identify plasma proteins differentiating placebo responders from non-responders, comprising immunoglobulins and proteins involved in oxidation reduction processes and complement activation. Plasma proteomics is a promising tool to identify molecular correlates and predictors of the placebo effect in humans.
format Online
Article
Text
id pubmed-7511022
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-75110222020-10-01 Molecular classification of the placebo effect in nausea Meissner, Karin Lutter, Dominik von Toerne, Christine Haile, Anja Woods, Stephen C. Hoffmann, Verena Ohmayer, Uli Hauck, Stefanie M. Tschoep, Matthias H. PLoS One Research Article In this proof-of-concept study, we tested whether placebo effects can be monitored and predicted by plasma proteins. In a randomized controlled design, 90 participants were exposed to a nauseating stimulus on two separate days and were randomly allocated to placebo treatment or no treatment on the second day. Significant placebo effects on nausea, motion sickness, and (in females) gastric activity could be verified. Using label-free tandem mass spectrometry, 74 differentially regulated proteins were identified as correlates of the placebo effect. Gene ontology (GO) enrichment analyses identified acute-phase proteins and microinflammatory proteins to be involved, and the identified GO signatures predicted day-adjusted scores of nausea indices in the placebo group. We also performed GO enrichment analyses of specific plasma proteins predictable by the experimental factors or their interactions and identified ‘grooming behavior’ as a prominent hit. Finally, Receiver Operator Characteristics (ROC) allowed to identify plasma proteins differentiating placebo responders from non-responders, comprising immunoglobulins and proteins involved in oxidation reduction processes and complement activation. Plasma proteomics is a promising tool to identify molecular correlates and predictors of the placebo effect in humans. Public Library of Science 2020-09-23 /pmc/articles/PMC7511022/ /pubmed/32966280 http://dx.doi.org/10.1371/journal.pone.0238533 Text en © 2020 Meissner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Meissner, Karin
Lutter, Dominik
von Toerne, Christine
Haile, Anja
Woods, Stephen C.
Hoffmann, Verena
Ohmayer, Uli
Hauck, Stefanie M.
Tschoep, Matthias H.
Molecular classification of the placebo effect in nausea
title Molecular classification of the placebo effect in nausea
title_full Molecular classification of the placebo effect in nausea
title_fullStr Molecular classification of the placebo effect in nausea
title_full_unstemmed Molecular classification of the placebo effect in nausea
title_short Molecular classification of the placebo effect in nausea
title_sort molecular classification of the placebo effect in nausea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511022/
https://www.ncbi.nlm.nih.gov/pubmed/32966280
http://dx.doi.org/10.1371/journal.pone.0238533
work_keys_str_mv AT meissnerkarin molecularclassificationoftheplaceboeffectinnausea
AT lutterdominik molecularclassificationoftheplaceboeffectinnausea
AT vontoernechristine molecularclassificationoftheplaceboeffectinnausea
AT haileanja molecularclassificationoftheplaceboeffectinnausea
AT woodsstephenc molecularclassificationoftheplaceboeffectinnausea
AT hoffmannverena molecularclassificationoftheplaceboeffectinnausea
AT ohmayeruli molecularclassificationoftheplaceboeffectinnausea
AT hauckstefaniem molecularclassificationoftheplaceboeffectinnausea
AT tschoepmatthiash molecularclassificationoftheplaceboeffectinnausea