Cargando…

Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing

BACKGROUND: The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) has prompted a need for mass testing to identify patients with viral infection. The high demand has created a global bottleneck in testing capacity, which prompted us to modify available resources to extract vir...

Descripción completa

Detalles Bibliográficos
Autores principales:	Al-Saud, Haya, Al-Romaih, Khaldoun, Bakheet, Razan, Mahmoud, Lina, Al-Harbi, Najla, Alshareef, Ibtihaj, Judia, Sara Bin, Aharbi, Layla, Alzayed, Abdulaziz, Jabaan, Amjad, Alhadrami, Hani, Albarrag, Ahmed, Azhar, Essam I., Al-Mozaini, Maha Ahmad
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	King Faisal Specialist Hospital and Research Centre 2020
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511039/ https://www.ncbi.nlm.nih.gov/pubmed/32954791 http://dx.doi.org/10.5144/0256-4947.2020.373

Ejemplares similares

Effect of gamma irradiation on filtering facepiece respirators and SARS-CoV-2 detection
por: Al-Hadyan, Khaled, et al.
Publicado: (2021)

Alterations in the Plasma Proteome Induced by SARS-CoV-2 and MERS-CoV Reveal Biomarkers for Disease Outcomes for COVID-19 Patients
por: Alaiya, Ayodele, et al.
Publicado: (2021)

HIV-2 Vpx neutralizes host restriction factor SAMHD1 to promote viral pathogenesis
por: Mohamed, Ahlam, et al.
Publicado: (2021)

Evaluation of two molecular detection platforms for gastroenteritis pathogens in treated sewage water in the Eastern province of Saudi Arabia
por: Al-Wohaib, Fawaz A., et al.
Publicado: (2022)

Establishing a Reference Dose–Response Calibration Curve for Dicentric Chromosome Aberrations to Assess Accidental Radiation Exposure in Saudi Arabia
por: Alsbeih, Ghazi A., et al.
Publicado: (2020)

Intracranial extension of an intramuscular haemangioma of superior rectus: case report and literature review
por: Al-Johani, Saud, et al.
Publicado: (2022)

Prevalence of Human Papillomavirus (HPV) Infection and the Association with Survival in Saudi Patients with Head and Neck Squamous Cell Carcinoma
por: Alsbeih, Ghazi, et al.
Publicado: (2019)

Multivariate piecewise linear regression model to predict radiosensitivity using the association with the genome-wide copy number variation
por: Tobiasz, Joanna, et al.
Publicado: (2023)

Favipiravir Effectiveness and Safety in Hospitalized Moderate-Severe COVID-19 Patients: Observational Prospective Multicenter Investigation in Saudi Arabia
por: Al-Muhsen, Saleh, et al.
Publicado: (2022)

Reduced rate of human papillomavirus infection and genetic overtransmission of TP53 72C polymorphic variant lower cervical cancer incidence
por: Alsbeih, Ghazi A., et al.
Publicado: (2017)

Glued Intraocular Lens Combined with Endothelial Keratoplasty: A Case Report
por: Al-Romaih, Arwa Z., et al.
Publicado: (2019)

Voltammetric-based immunosensor for the detection of SARS-CoV-2 nucleocapsid antigen
por: Eissa, Shimaa, et al.
Publicado: (2021)

Assessing the knowledge and attitude towards HIV/AIDS among the general population and health care professionals in MENA region
por: Elghazaly, Ayman, et al.
Publicado: (2023)

Headspace Solid Phase Micro-Extraction of Volatile Constituents Produced from Saudi Ruta chalepensis and Molecular Docking Study of Potential Antioxidant Activity
por: Aati, Hanan Y., et al.
Publicado: (2023)

Human Kidney Disease-causing INF2 Mutations Perturb Rho/Dia Signaling in the Glomerulus
por: Sun, Hua, et al.
Publicado: (2014)

In Vitro Analysis of Integrated Global High-Resolution DNA Methylation Profiling with Genomic Imbalance and Gene Expression in Osteosarcoma
por: Sadikovic, Bekim, et al.
Publicado: (2008)

SARS-CoV-2 Reinfection Rate and Outcomes in Saudi Arabia: A National Retrospective Study
por: Al-Otaiby, Maram, et al.
Publicado: (2022)

Parental acceptance of silver Diamine fluoride application on primary dentition: a systematic review and meta-analysis
por: Sabbagh, Heba, et al.
Publicado: (2020)

In Vitro Antibacterial Effects of Five Volatile Oil Extracts Against Intramacrophage Brucella Abortus 544
por: Al-Mariri, Ayman, et al.
Publicado: (2012)

Association of Electronic Media Use and Sleep Habits Among Secondary School Students in Al-Madinah
por: Al-Anazi, Nojood Saud, et al.
Publicado: (2022)

Immunological and Histological Studies of Different Concentrations of Rosmarinus officinalis and Thymus vulgaris Extracts on Thymus Gland of Chick Embryos
por: Alzahri, Reem Yahya, et al.
Publicado: (2023)

A successful endoscopic extraction of accidently ingested toothbrush in an adult: a case report
por: Al-Mulla, Ahmad Essam, et al.
Publicado: (2023)

Appropriateness of upper gastrointestinal endoscopy referrals from primary health care
por: Al-Romaih, Wafa R., et al.
Publicado: (2006)

Inhibitory Activity of Illicium verum Extracts against Avian Viruses
por: Alhajj, Mohammed S., et al.
Publicado: (2020)

Influence of inflammation on bleeding and wound healing following surgical extraction of impacted lower third molars
por: Cheng, Yusheng, et al.
Publicado: (2023)

ZNFX1 Deficiency in a Child with Interstitial Pneumonitis and Peripheral Monocytosis
por: Al-Saud, Bandar, et al.
Publicado: (2023)

The value of renal ultrasound in children with a first episode of urinary tract infection
por: Alshamsan, Layla, et al.
Publicado: (2009)

Effect of ginseng extract on the TGF-β1 signaling pathway in CCl(4)-induced liver fibrosis in rats
por: Hafez, Mohamed M., et al.
Publicado: (2017)

Potential antiviral agents of Rosmarinus officinalis extract against herpes viruses 1 and 2
por: AL-Megrin, Wafa A., et al.
Publicado: (2020)

HPV prevalence and genetic predisposition to cervical cancer in Saudi Arabia
por: Alsbeih, Ghazi, et al.
Publicado: (2013)

Linezolid vs Vancomycin in Induced Thrombocytopenia
por: Al-Harbi, Dimah, et al.
Publicado: (2022)

Functional outcomes in bilateral upper limb Amelia patient with scoliosis post vertical expandable prosthetic titanium rib (VEPTR) application: A case report
por: AlMarshad, Abdullah, et al.
Publicado: (2020)

Clinical characteristics, risk factors, and rate of severity of a nationwide COVID-19 Saudi cohort
por: Al-Numair, Nouf S., et al.
Publicado: (2022)

Comparative Study for Spectrofluorimetric Determination of Ambroxol Hydrochloride Using Aluminum Metal Transfer Chelation Complex and Biogenic Synthesis of Aluminum Oxide Nanoparticles Using Lavandula spica Flowers Extract
por: Al-Humud, Najla S., et al.
Publicado: (2023)

Deep Eutectic Solvent-Based Microextraction of Lead(II) Traces from Water and Aqueous Extracts before FAAS Measurements
por: Habila, Mohamed A., et al.
Publicado: (2020)

Molecular analysis of T(-)B(-)NK(+ )severe combined immunodeficiency and Omenn syndrome cases in Saudi Arabia
por: Alsmadi, Osama, et al.
Publicado: (2009)

Pediatric Tracheostomy: A Quality-of-Life Assessment Study in Saudi Arabia
por: Al-Faleh, Moath, et al.
Publicado: (2023)

Impaired DNA Repair Fidelity in a Breast Cancer Patient With Adverse Reactions to Radiotherapy
por: Alsbeih, Ghazi, et al.
Publicado: (2021)

Choice of specimen’s extraction site affects wound morbidity in laparoscopic colorectal cancer surgery
por: Al Dhaheri, Mahmood, et al.
Publicado: (2022)

Partner in fat metabolism: role of KLFs in fat burning and reproductive behavior
por: Hashmi, Sarwar, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_rj482fmo04rp7933npf2na5elu