Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number

Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (Re(L)) and the k(L)a coefficient, as key parameters characterized the bioprocessing of HL-60 cells in ReadyToProcess WAVE(TM) 25 system, on reached values of the apparent maximal specific growth rate (μ(max)) and the specific yield of biomass (Y(*)(X/S)) has been identified. The values of Re(L) (i.e., 510–10,208), as well as k(L)a coefficient (i.e., 2.83–13.55 h(−1)), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparent μ(max) = 0.038 h(−1) and Y(*)(X/S) = 25.64 × 10(8) cells g(glc)(−1) have been noted for cultures independently performed at wave-induced agitation characterized by Re(L) equaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude.