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Direct imaging of antigen–antibody binding by atomic force microscopy
Direct observation of antigen–antibody binding at the nanoscale has always been a considerable challenging problem, and researchers have made tremendous efforts on it. In this study, the morphology of biotinylated antibody-specific Immunoglobulin E (IgE) immune complexes has been successfully imaged...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511526/ https://www.ncbi.nlm.nih.gov/pubmed/32989412 http://dx.doi.org/10.1007/s13204-020-01558-w |
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author | Hu, Jing Gao, Mingyan Wang, Zuobin Chen, Yujuan Song, Zhengxun Xu, Hongmei |
author_facet | Hu, Jing Gao, Mingyan Wang, Zuobin Chen, Yujuan Song, Zhengxun Xu, Hongmei |
author_sort | Hu, Jing |
collection | PubMed |
description | Direct observation of antigen–antibody binding at the nanoscale has always been a considerable challenging problem, and researchers have made tremendous efforts on it. In this study, the morphology of biotinylated antibody-specific Immunoglobulin E (IgE) immune complexes has been successfully imaged by atomic force microscopy (AFM) in the tapping-mode. The AFM images indicated that the individual immune complex was composed of an IgE and a biotinylated antibody. Excitingly, it is the first time that we have actually seen the IgE binding to biotinylated antibody. Alternatively, information on the length of IgE, biotinylated antibodies and biotinylated antibody-specific IgE immune complexes were also obtained, respectively. These results indicate the versatility of AFM technology in the identification of antigen–antibody binding. This work not only lays the basis for the direct imaging of the biotinylated antibody-IgE by AFM, but also offers valuable information for studying the targeted therapy and vaccine development in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13204-020-01558-w) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7511526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-75115262020-09-24 Direct imaging of antigen–antibody binding by atomic force microscopy Hu, Jing Gao, Mingyan Wang, Zuobin Chen, Yujuan Song, Zhengxun Xu, Hongmei Appl Nanosci Original Article Direct observation of antigen–antibody binding at the nanoscale has always been a considerable challenging problem, and researchers have made tremendous efforts on it. In this study, the morphology of biotinylated antibody-specific Immunoglobulin E (IgE) immune complexes has been successfully imaged by atomic force microscopy (AFM) in the tapping-mode. The AFM images indicated that the individual immune complex was composed of an IgE and a biotinylated antibody. Excitingly, it is the first time that we have actually seen the IgE binding to biotinylated antibody. Alternatively, information on the length of IgE, biotinylated antibodies and biotinylated antibody-specific IgE immune complexes were also obtained, respectively. These results indicate the versatility of AFM technology in the identification of antigen–antibody binding. This work not only lays the basis for the direct imaging of the biotinylated antibody-IgE by AFM, but also offers valuable information for studying the targeted therapy and vaccine development in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13204-020-01558-w) contains supplementary material, which is available to authorized users. Springer International Publishing 2020-09-24 2021 /pmc/articles/PMC7511526/ /pubmed/32989412 http://dx.doi.org/10.1007/s13204-020-01558-w Text en © King Abdulaziz City for Science and Technology 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Hu, Jing Gao, Mingyan Wang, Zuobin Chen, Yujuan Song, Zhengxun Xu, Hongmei Direct imaging of antigen–antibody binding by atomic force microscopy |
title | Direct imaging of antigen–antibody binding by atomic force microscopy |
title_full | Direct imaging of antigen–antibody binding by atomic force microscopy |
title_fullStr | Direct imaging of antigen–antibody binding by atomic force microscopy |
title_full_unstemmed | Direct imaging of antigen–antibody binding by atomic force microscopy |
title_short | Direct imaging of antigen–antibody binding by atomic force microscopy |
title_sort | direct imaging of antigen–antibody binding by atomic force microscopy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511526/ https://www.ncbi.nlm.nih.gov/pubmed/32989412 http://dx.doi.org/10.1007/s13204-020-01558-w |
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