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A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay
While industrial nitrogen fertilizer is intrinsic to modern agriculture, it is expensive and environmentally harmful. One approach to reduce fertilizer usage is to engineer the bacterial nitrogenase enzyme complex within plant mitochondria, a location that may support enzyme function. Our current st...
Autores principales: | Okada, Shoko, Gregg, Christina M., Allen, Robert Silas, Menon, Amratha, Hussain, Dawar, Gillespie, Vanessa, Johnston, Ema, Byrne, Keren, Colgrave, Michelle Lisa, Wood, Craig C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511584/ https://www.ncbi.nlm.nih.gov/pubmed/33013970 http://dx.doi.org/10.3389/fpls.2020.552160 |
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