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miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4
Gliomas are the most common malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotypes. MicroRNAs (miRNAs) play a regulatory role in various cancers, including gliomas; however, their specific roles and mechanisms have not been fully investigated. Studies hav...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511594/ https://www.ncbi.nlm.nih.gov/pubmed/33014873 http://dx.doi.org/10.3389/fonc.2020.01772 |
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author | Dai, Yong Chen, Zhenhua Zhao, Wei Cai, Gang Wang, Zhifeng Wang, Xuejiang Hu, Hongkang Zhang, Yi |
author_facet | Dai, Yong Chen, Zhenhua Zhao, Wei Cai, Gang Wang, Zhifeng Wang, Xuejiang Hu, Hongkang Zhang, Yi |
author_sort | Dai, Yong |
collection | PubMed |
description | Gliomas are the most common malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotypes. MicroRNAs (miRNAs) play a regulatory role in various cancers, including gliomas; however, their specific roles and mechanisms have not been fully investigated. Studies have indicated that miR-29a is a tumor-suppressive miRNA, but the data are limited. In this study, we investigated the role of miR-29a-5p in glioma and further explored its underlying mechanisms. On the basis of bioinformatics, dehydrogenase/reductase 4 (DHRS4) was considered a potential target of miR-29a-5p and was also found to be highly expressed in gliomas in our experiments. Moreover, with a luciferase reporter assay, DHRS4 was found to be a target gene of miR-29a-5p and to be correlated with glioma proliferation, invasion, and migration in our in vivo and in vitro experiments. Simultaneously, we observed that the knockdown of DHRS4 rescued the downregulation of glioma proliferation, invasion, and migration caused by treatment with a mir-29a-5p inhibitor. The present findings demonstrate that miR-29a-5p suppresses cell proliferation, invasion, and migration by targeting DHRS4, and DHRS4 may be a potential new oncogene and prognostic factor in gliomas. |
format | Online Article Text |
id | pubmed-7511594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75115942020-10-02 miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 Dai, Yong Chen, Zhenhua Zhao, Wei Cai, Gang Wang, Zhifeng Wang, Xuejiang Hu, Hongkang Zhang, Yi Front Oncol Oncology Gliomas are the most common malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotypes. MicroRNAs (miRNAs) play a regulatory role in various cancers, including gliomas; however, their specific roles and mechanisms have not been fully investigated. Studies have indicated that miR-29a is a tumor-suppressive miRNA, but the data are limited. In this study, we investigated the role of miR-29a-5p in glioma and further explored its underlying mechanisms. On the basis of bioinformatics, dehydrogenase/reductase 4 (DHRS4) was considered a potential target of miR-29a-5p and was also found to be highly expressed in gliomas in our experiments. Moreover, with a luciferase reporter assay, DHRS4 was found to be a target gene of miR-29a-5p and to be correlated with glioma proliferation, invasion, and migration in our in vivo and in vitro experiments. Simultaneously, we observed that the knockdown of DHRS4 rescued the downregulation of glioma proliferation, invasion, and migration caused by treatment with a mir-29a-5p inhibitor. The present findings demonstrate that miR-29a-5p suppresses cell proliferation, invasion, and migration by targeting DHRS4, and DHRS4 may be a potential new oncogene and prognostic factor in gliomas. Frontiers Media S.A. 2020-09-10 /pmc/articles/PMC7511594/ /pubmed/33014873 http://dx.doi.org/10.3389/fonc.2020.01772 Text en Copyright © 2020 Dai, Chen, Zhao, Cai, Wang, Wang, Hu and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Dai, Yong Chen, Zhenhua Zhao, Wei Cai, Gang Wang, Zhifeng Wang, Xuejiang Hu, Hongkang Zhang, Yi miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title | miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title_full | miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title_fullStr | miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title_full_unstemmed | miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title_short | miR-29a-5p Regulates the Proliferation, Invasion, and Migration of Gliomas by Targeting DHRS4 |
title_sort | mir-29a-5p regulates the proliferation, invasion, and migration of gliomas by targeting dhrs4 |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511594/ https://www.ncbi.nlm.nih.gov/pubmed/33014873 http://dx.doi.org/10.3389/fonc.2020.01772 |
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