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Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis

The microbial communities inhabiting the fronds of duckweeds have not been investigated in as much detail as those on the roots. We herein examined the microbial communities in three duckweed species using 16S rRNA amplicon sequencing and compared them to those on the roots. The microbial compositio...

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Autores principales: Iwashita, Tomoki, Tanaka, Yasuhiro, Tamaki, Hideyuki, Yoneda, Yasuko, Makino, Ayaka, Tateno, Yuka, Li, Yan, Toyama, Tadashi, Kamagata, Yoichi, Mori, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511783/
https://www.ncbi.nlm.nih.gov/pubmed/32684532
http://dx.doi.org/10.1264/jsme2.ME20081
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author Iwashita, Tomoki
Tanaka, Yasuhiro
Tamaki, Hideyuki
Yoneda, Yasuko
Makino, Ayaka
Tateno, Yuka
Li, Yan
Toyama, Tadashi
Kamagata, Yoichi
Mori, Kazuhiro
author_facet Iwashita, Tomoki
Tanaka, Yasuhiro
Tamaki, Hideyuki
Yoneda, Yasuko
Makino, Ayaka
Tateno, Yuka
Li, Yan
Toyama, Tadashi
Kamagata, Yoichi
Mori, Kazuhiro
author_sort Iwashita, Tomoki
collection PubMed
description The microbial communities inhabiting the fronds of duckweeds have not been investigated in as much detail as those on the roots. We herein examined the microbial communities in three duckweed species using 16S rRNA amplicon sequencing and compared them to those on the roots. The microbial compositions of the fronds were distinct from those of the roots in the three species. Various types of taxonomic bacteria, including rarely cultivated phyla, Acidobacteria, Armatimonadetes, and Verrucomicrobia, were also isolated from the fronds, but at a slightly lower abundance than those from the roots. These results suggest that duckweed fronds are an alternative source for isolating rare and novel microbes, which may otherwise be recalcitrant to cultivation using conventional strategies.
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spelling pubmed-75117832020-11-18 Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis Iwashita, Tomoki Tanaka, Yasuhiro Tamaki, Hideyuki Yoneda, Yasuko Makino, Ayaka Tateno, Yuka Li, Yan Toyama, Tadashi Kamagata, Yoichi Mori, Kazuhiro Microbes Environ Short Communication The microbial communities inhabiting the fronds of duckweeds have not been investigated in as much detail as those on the roots. We herein examined the microbial communities in three duckweed species using 16S rRNA amplicon sequencing and compared them to those on the roots. The microbial compositions of the fronds were distinct from those of the roots in the three species. Various types of taxonomic bacteria, including rarely cultivated phyla, Acidobacteria, Armatimonadetes, and Verrucomicrobia, were also isolated from the fronds, but at a slightly lower abundance than those from the roots. These results suggest that duckweed fronds are an alternative source for isolating rare and novel microbes, which may otherwise be recalcitrant to cultivation using conventional strategies. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020 2020-07-17 /pmc/articles/PMC7511783/ /pubmed/32684532 http://dx.doi.org/10.1264/jsme2.ME20081 Text en 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Iwashita, Tomoki
Tanaka, Yasuhiro
Tamaki, Hideyuki
Yoneda, Yasuko
Makino, Ayaka
Tateno, Yuka
Li, Yan
Toyama, Tadashi
Kamagata, Yoichi
Mori, Kazuhiro
Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title_full Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title_fullStr Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title_full_unstemmed Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title_short Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis
title_sort comparative analysis of microbial communities in fronds and roots of three duckweed species: spirodela polyrhiza, lemna minor, and lemna aequinoctialis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511783/
https://www.ncbi.nlm.nih.gov/pubmed/32684532
http://dx.doi.org/10.1264/jsme2.ME20081
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