Synthesis of arylidene hydrazinylpyrido[2,3-d]pyrimidin-4-ones as potent anti-microbial agents

Combination of arylidene hydrazinyl moiety with pyrido[2,3-d]pyrimidin-4-one skeleton in compounds 7‒26 results in the output of unprecedented anti-microbial agents. Arylidene hydrazinyl based on Pyrido[2,3-d]pyrimidin-4-one analoges 7‒26 prepared by the treatment of [2,3-d]pyrimidin-4-ones 6a,b with various aromatic aldehydes. The antimicrobial action for recently synthesized compounds was considered towards gram positive bacterial species (Staphylococcus aurous ATCC- 47077; Bacillus cereus ATCC-12228), gram negative bacterial species (Escherichia coli ATCC-25922; Salmonella typhi ATCC-15566) and Candida albicans ATCC-10231 as fungal strains. The antimicrobial action expanded by expanding the electron donating group in position 2 and 5 for Pyrido[2,3-d]pyrimidin-4-one core. Derivatives 13, 14, 15, 16 and 12; individually appeared hopeful anti-microbial action towards all strains utilized with inhibition zone higher than that of standard reference drug with lowest MIC.