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Magnetic Iron Nanocubes Effectively Capture Epithelial and Mesenchymal Cancer Cells
[Image: see text] Current methods for capturing circulating tumor cells (CTCs) are based on the overexpression of cytokeratin (CK) or epithelial cell-adhesion molecule (EpCAM) on cancer cells. However, during the process of metastasis, tumor cells undergo epithelial-to-mesenchymal transition (EMT) t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513327/ https://www.ncbi.nlm.nih.gov/pubmed/32984691 http://dx.doi.org/10.1021/acsomega.0c02699 |
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author | Suresh, Dhananjay Ghoshdastidar, Shreya Gangula, Abilash Mukherjee, Soumavo Upendran, Anandhi Kannan, Raghuraman |
author_facet | Suresh, Dhananjay Ghoshdastidar, Shreya Gangula, Abilash Mukherjee, Soumavo Upendran, Anandhi Kannan, Raghuraman |
author_sort | Suresh, Dhananjay |
collection | PubMed |
description | [Image: see text] Current methods for capturing circulating tumor cells (CTCs) are based on the overexpression of cytokeratin (CK) or epithelial cell-adhesion molecule (EpCAM) on cancer cells. However, during the process of metastasis, tumor cells undergo epithelial-to-mesenchymal transition (EMT) that can lead to the loss of CK/EpCAM expression. Therefore, it is vital to develop a capturing technique independent of CK/EpCAM expression on the cancer cell. To develop this technique, it is important to identify common secondary oncogenic markers overexpressed on tumor cells before and after EMT. We analyzed the biomarker expression levels in tumor cells, before and after EMT, and found two common proteins—human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) whose levels remained unaffected. So, we synthesized immunomagnetic iron nanocubes covalently conjugated with antibodies of Her2 or EGFR to capture cancer cells irrespective of the EMT status. The nanocubes showed high specificity (6–9-fold) in isolating the cancer cells of interest from a mixture of cells spiked in serum. We characterized the captured cells for identifying their EMT status. Thus, we believe the results presented here would help in the development of novel strategies for capturing both primary and metastatic cancer cells from patients’ blood to develop an effective treatment plan. |
format | Online Article Text |
id | pubmed-7513327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-75133272020-09-25 Magnetic Iron Nanocubes Effectively Capture Epithelial and Mesenchymal Cancer Cells Suresh, Dhananjay Ghoshdastidar, Shreya Gangula, Abilash Mukherjee, Soumavo Upendran, Anandhi Kannan, Raghuraman ACS Omega [Image: see text] Current methods for capturing circulating tumor cells (CTCs) are based on the overexpression of cytokeratin (CK) or epithelial cell-adhesion molecule (EpCAM) on cancer cells. However, during the process of metastasis, tumor cells undergo epithelial-to-mesenchymal transition (EMT) that can lead to the loss of CK/EpCAM expression. Therefore, it is vital to develop a capturing technique independent of CK/EpCAM expression on the cancer cell. To develop this technique, it is important to identify common secondary oncogenic markers overexpressed on tumor cells before and after EMT. We analyzed the biomarker expression levels in tumor cells, before and after EMT, and found two common proteins—human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) whose levels remained unaffected. So, we synthesized immunomagnetic iron nanocubes covalently conjugated with antibodies of Her2 or EGFR to capture cancer cells irrespective of the EMT status. The nanocubes showed high specificity (6–9-fold) in isolating the cancer cells of interest from a mixture of cells spiked in serum. We characterized the captured cells for identifying their EMT status. Thus, we believe the results presented here would help in the development of novel strategies for capturing both primary and metastatic cancer cells from patients’ blood to develop an effective treatment plan. American Chemical Society 2020-09-09 /pmc/articles/PMC7513327/ /pubmed/32984691 http://dx.doi.org/10.1021/acsomega.0c02699 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Suresh, Dhananjay Ghoshdastidar, Shreya Gangula, Abilash Mukherjee, Soumavo Upendran, Anandhi Kannan, Raghuraman Magnetic Iron Nanocubes Effectively Capture Epithelial and Mesenchymal Cancer Cells |
title | Magnetic Iron Nanocubes Effectively Capture Epithelial
and Mesenchymal Cancer Cells |
title_full | Magnetic Iron Nanocubes Effectively Capture Epithelial
and Mesenchymal Cancer Cells |
title_fullStr | Magnetic Iron Nanocubes Effectively Capture Epithelial
and Mesenchymal Cancer Cells |
title_full_unstemmed | Magnetic Iron Nanocubes Effectively Capture Epithelial
and Mesenchymal Cancer Cells |
title_short | Magnetic Iron Nanocubes Effectively Capture Epithelial
and Mesenchymal Cancer Cells |
title_sort | magnetic iron nanocubes effectively capture epithelial
and mesenchymal cancer cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513327/ https://www.ncbi.nlm.nih.gov/pubmed/32984691 http://dx.doi.org/10.1021/acsomega.0c02699 |
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