Cargando…
Action of a minimal contractile bactericidal nanomachine
R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1–4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513463/ https://www.ncbi.nlm.nih.gov/pubmed/32350467 http://dx.doi.org/10.1038/s41586-020-2186-z |
_version_ | 1783586389651095552 |
---|---|
author | Ge, Peng Scholl, Dean Prokhorov, Nikolai S. Avaylon, Jaycob Shneider, Mikhail M. Browning, Chris Buth, Sergii A. Plattner, Michel Chakraborty, Urmi Ding, Ke Leiman, Petr G. Miller, Jeff F. Zhou, Z. Hong |
author_facet | Ge, Peng Scholl, Dean Prokhorov, Nikolai S. Avaylon, Jaycob Shneider, Mikhail M. Browning, Chris Buth, Sergii A. Plattner, Michel Chakraborty, Urmi Ding, Ke Leiman, Petr G. Miller, Jeff F. Zhou, Z. Hong |
author_sort | Ge, Peng |
collection | PubMed |
description | R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1–4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of (DNA-containing) T4 bacteriophage5. Here we report the atomic model of the complete R2 pyocin in its pre- and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the sequence of events during pyocin contraction: tail fibers trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium. |
format | Online Article Text |
id | pubmed-7513463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-75134632020-10-15 Action of a minimal contractile bactericidal nanomachine Ge, Peng Scholl, Dean Prokhorov, Nikolai S. Avaylon, Jaycob Shneider, Mikhail M. Browning, Chris Buth, Sergii A. Plattner, Michel Chakraborty, Urmi Ding, Ke Leiman, Petr G. Miller, Jeff F. Zhou, Z. Hong Nature Article R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1–4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of (DNA-containing) T4 bacteriophage5. Here we report the atomic model of the complete R2 pyocin in its pre- and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the sequence of events during pyocin contraction: tail fibers trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium. 2020-04-15 2020-04 /pmc/articles/PMC7513463/ /pubmed/32350467 http://dx.doi.org/10.1038/s41586-020-2186-z Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Ge, Peng Scholl, Dean Prokhorov, Nikolai S. Avaylon, Jaycob Shneider, Mikhail M. Browning, Chris Buth, Sergii A. Plattner, Michel Chakraborty, Urmi Ding, Ke Leiman, Petr G. Miller, Jeff F. Zhou, Z. Hong Action of a minimal contractile bactericidal nanomachine |
title | Action of a minimal contractile bactericidal nanomachine |
title_full | Action of a minimal contractile bactericidal nanomachine |
title_fullStr | Action of a minimal contractile bactericidal nanomachine |
title_full_unstemmed | Action of a minimal contractile bactericidal nanomachine |
title_short | Action of a minimal contractile bactericidal nanomachine |
title_sort | action of a minimal contractile bactericidal nanomachine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513463/ https://www.ncbi.nlm.nih.gov/pubmed/32350467 http://dx.doi.org/10.1038/s41586-020-2186-z |
work_keys_str_mv | AT gepeng actionofaminimalcontractilebactericidalnanomachine AT scholldean actionofaminimalcontractilebactericidalnanomachine AT prokhorovnikolais actionofaminimalcontractilebactericidalnanomachine AT avaylonjaycob actionofaminimalcontractilebactericidalnanomachine AT shneidermikhailm actionofaminimalcontractilebactericidalnanomachine AT browningchris actionofaminimalcontractilebactericidalnanomachine AT buthsergiia actionofaminimalcontractilebactericidalnanomachine AT plattnermichel actionofaminimalcontractilebactericidalnanomachine AT chakrabortyurmi actionofaminimalcontractilebactericidalnanomachine AT dingke actionofaminimalcontractilebactericidalnanomachine AT leimanpetrg actionofaminimalcontractilebactericidalnanomachine AT millerjefff actionofaminimalcontractilebactericidalnanomachine AT zhouzhong actionofaminimalcontractilebactericidalnanomachine |