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BioBrick-based ‘Quick Gene Assembly’ in vitro
Because of the technological limitations of de novo DNA synthesis in (i) making constructs containing tandemly repeated DNA sequence units, (ii) making an unbiased DNA library containing DNA fragments with sequence multiplicity in a specific region of target genes, and (iii) replacing DNA fragments,...
Autores principales: | Yamazaki, Ken-ichi, de Mora, Kim, Saitoh, Kensuke |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513740/ https://www.ncbi.nlm.nih.gov/pubmed/32995504 http://dx.doi.org/10.1093/synbio/ysx003 |
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