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Multiplex in situ hybridization within a single transcript: RNAscope reveals dystrophin mRNA dynamics

Dystrophin plays a vital role in maintaining muscle health, yet low mRNA expression, lengthy transcription time and the limitations of traditional in-situ hybridization (ISH) methodologies mean that the dynamics of dystrophin transcription remain poorly understood. RNAscope is highly sensitive ISH m...

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Detalles Bibliográficos
Autores principales: Hildyard, John C. W., Rawson, Faye, Wells, Dominic J., Piercy, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514052/
https://www.ncbi.nlm.nih.gov/pubmed/32970731
http://dx.doi.org/10.1371/journal.pone.0239467