Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A

We have generated an induced pluripotent stem cell (iPSC) line KCLi001-A (iOP118) from a female atopic dermatitis (AD) patient, heterozygous for the loss-of-function mutation c.2282del4 in the filaggrin gene (FLG). Epidermal keratinocytes were reprogrammed using non-integrating Sendai virus vectors....

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Autores principales: Devito, Liani, Donne, Matthew, Kolundzic, Nikola, Khurana, Preeti, Hobbs, Carl, Kaddour, Gabriel, Dubrac, Sandrine, Gruber, Robert, Schmuth, Matthias, Mauro, Thea, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514110/
https://www.ncbi.nlm.nih.gov/pubmed/30075366
http://dx.doi.org/10.1016/j.scr.2018.07.014
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author Devito, Liani
Donne, Matthew
Kolundzic, Nikola
Khurana, Preeti
Hobbs, Carl
Kaddour, Gabriel
Dubrac, Sandrine
Gruber, Robert
Schmuth, Matthias
Mauro, Thea
Ilic, Dusko
author_facet Devito, Liani
Donne, Matthew
Kolundzic, Nikola
Khurana, Preeti
Hobbs, Carl
Kaddour, Gabriel
Dubrac, Sandrine
Gruber, Robert
Schmuth, Matthias
Mauro, Thea
Ilic, Dusko
author_sort Devito, Liani
collection PubMed
description We have generated an induced pluripotent stem cell (iPSC) line KCLi001-A (iOP118) from a female atopic dermatitis (AD) patient, heterozygous for the loss-of-function mutation c.2282del4 in the filaggrin gene (FLG). Epidermal keratinocytes were reprogrammed using non-integrating Sendai virus vectors. The entire process of derivation and expansion of AD-iPSCs were performed under xeno-free culture conditions. Characterization of KCLi001-A line included molecular karyotyping, mutation screening using restriction enzyme digestion and Sanger sequencing, while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay.
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spelling pubmed-75141102020-09-24 Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A Devito, Liani Donne, Matthew Kolundzic, Nikola Khurana, Preeti Hobbs, Carl Kaddour, Gabriel Dubrac, Sandrine Gruber, Robert Schmuth, Matthias Mauro, Thea Ilic, Dusko Stem Cell Res Article We have generated an induced pluripotent stem cell (iPSC) line KCLi001-A (iOP118) from a female atopic dermatitis (AD) patient, heterozygous for the loss-of-function mutation c.2282del4 in the filaggrin gene (FLG). Epidermal keratinocytes were reprogrammed using non-integrating Sendai virus vectors. The entire process of derivation and expansion of AD-iPSCs were performed under xeno-free culture conditions. Characterization of KCLi001-A line included molecular karyotyping, mutation screening using restriction enzyme digestion and Sanger sequencing, while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay. 2018-07-25 2018-08 /pmc/articles/PMC7514110/ /pubmed/30075366 http://dx.doi.org/10.1016/j.scr.2018.07.014 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Devito, Liani
Donne, Matthew
Kolundzic, Nikola
Khurana, Preeti
Hobbs, Carl
Kaddour, Gabriel
Dubrac, Sandrine
Gruber, Robert
Schmuth, Matthias
Mauro, Thea
Ilic, Dusko
Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title_full Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title_fullStr Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title_full_unstemmed Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title_short Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
title_sort induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: kcli001-a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514110/
https://www.ncbi.nlm.nih.gov/pubmed/30075366
http://dx.doi.org/10.1016/j.scr.2018.07.014
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