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Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process

The purpose of this study is to introduce an improved Luedeking–Piret model that represents a structurally simple biomass concentration approach. The developed routine provides acceptable accuracy when fitting experimental data that incorporate the target protein concentration of Escherichia coli cu...

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Detalles Bibliográficos
Autores principales: Urniezius, Renaldas, Survyla, Arnas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514566/
http://dx.doi.org/10.3390/e21121221
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author Urniezius, Renaldas
Survyla, Arnas
author_facet Urniezius, Renaldas
Survyla, Arnas
author_sort Urniezius, Renaldas
collection PubMed
description The purpose of this study is to introduce an improved Luedeking–Piret model that represents a structurally simple biomass concentration approach. The developed routine provides acceptable accuracy when fitting experimental data that incorporate the target protein concentration of Escherichia coli culture BL21 (DE3) pET28a in fed-batch processes. This paper presents system identification, biomass, and product parameter fitting routines, starting from their roots of origin to the entropy-related development, characterized by robustness and simplicity. A single tuning coefficient allows for the selection of an optimization criterion that serves equally well for higher and lower biomass concentrations. The idea of the paper is to demonstrate that the use of fundamental knowledge can make the general model more common for technological use compared to a sophisticated artificial neural network. Experimental validation of the proposed model involved data analysis of six cultivation experiments compared to 19 experiments used for model fitting and parameter estimation.
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spelling pubmed-75145662020-11-09 Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process Urniezius, Renaldas Survyla, Arnas Entropy (Basel) Article The purpose of this study is to introduce an improved Luedeking–Piret model that represents a structurally simple biomass concentration approach. The developed routine provides acceptable accuracy when fitting experimental data that incorporate the target protein concentration of Escherichia coli culture BL21 (DE3) pET28a in fed-batch processes. This paper presents system identification, biomass, and product parameter fitting routines, starting from their roots of origin to the entropy-related development, characterized by robustness and simplicity. A single tuning coefficient allows for the selection of an optimization criterion that serves equally well for higher and lower biomass concentrations. The idea of the paper is to demonstrate that the use of fundamental knowledge can make the general model more common for technological use compared to a sophisticated artificial neural network. Experimental validation of the proposed model involved data analysis of six cultivation experiments compared to 19 experiments used for model fitting and parameter estimation. MDPI 2019-12-14 /pmc/articles/PMC7514566/ http://dx.doi.org/10.3390/e21121221 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Urniezius, Renaldas
Survyla, Arnas
Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title_full Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title_fullStr Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title_full_unstemmed Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title_short Identification of Functional Bioprocess Model for Recombinant E. Coli Cultivation Process
title_sort identification of functional bioprocess model for recombinant e. coli cultivation process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514566/
http://dx.doi.org/10.3390/e21121221
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AT survylaarnas identificationoffunctionalbioprocessmodelforrecombinantecolicultivationprocess