PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues

CRISPR/Cas9 functional genomic screens have emerged as essential tools in drug target discovery. However, the sensitivity of available genome-wide CRISPR libraries is impaired by guides which inefficiently abrogate gene function. While Cas9 cleavage efficiency optimization and essential domain targe...

Descripción completa

Detalles Bibliográficos
Autores principales: Veeneman, Brendan, Gao, Ying, Grant, Joy, Fruhling, David, Ahn, James, Bosbach, Benedikt, Bienkowska, Jadwiga, Follettie, Maximillian, Arndt, Kim, Myers, Jeremy, Zhong, Wenyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Computational Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515706/
https://www.ncbi.nlm.nih.gov/pubmed/32821942
http://dx.doi.org/10.1093/nar/gkaa645

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515706/
https://www.ncbi.nlm.nih.gov/pubmed/32821942
http://dx.doi.org/10.1093/nar/gkaa645

Ejemplares similares