Cargando…

PINCER: improved CRISPR/Cas9 screening by efficient cleavage at conserved residues

CRISPR/Cas9 functional genomic screens have emerged as essential tools in drug target discovery. However, the sensitivity of available genome-wide CRISPR libraries is impaired by guides which inefficiently abrogate gene function. While Cas9 cleavage efficiency optimization and essential domain targe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Veeneman, Brendan, Gao, Ying, Grant, Joy, Fruhling, David, Ahn, James, Bosbach, Benedikt, Bienkowska, Jadwiga, Follettie, Maximillian, Arndt, Kim, Myers, Jeremy, Zhong, Wenyan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Computational Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515706/ https://www.ncbi.nlm.nih.gov/pubmed/32821942 http://dx.doi.org/10.1093/nar/gkaa645

Ejemplares similares

Programmable RNA recognition and cleavage by CRISPR/Cas9
por: O’Connell, Mitchell R., et al.
Publicado: (2014)

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

Conformational control of DNA target cleavage by CRISPR–Cas9
por: Sternberg, Samuel H., et al.
Publicado: (2015)

Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo
por: Yarrington, Robert M., et al.
Publicado: (2018)

Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics
por: Lv, Hailong, et al.
Publicado: (2021)

crisprSQL: a novel database platform for CRISPR/Cas off-target cleavage assays
por: Störtz, Florian, et al.
Publicado: (2020)

Cleavage of Phage DNA by the Streptococcus thermophilus CRISPR3-Cas System
por: Magadán, Alfonso H., et al.
Publicado: (2012)

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
por: Liu, Xiaoxi, et al.
Publicado: (2016)

Structural insights into DNA cleavage activation of CRISPR-Cas9 system
por: Huai, Cong, et al.
Publicado: (2017)

Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells
por: Tang, Lianchao, et al.
Publicado: (2019)

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage
por: Sun, Jichao, et al.
Publicado: (2019)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
por: Jeon, Yongmoon, et al.
Publicado: (2018)

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
por: Dagdas, Yavuz S., et al.
Publicado: (2017)

Frequent Aneuploidy in Primary Human T Cells after CRISPR-Cas9 cleavage
por: Nahmad, A.D., et al.
Publicado: (2022)

Element coding based accurate evaluation of CRISPR/Cas9 initial cleavage
por: Hu, Jianyu, et al.
Publicado: (2021)

CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes
por: Schelling, Michael A., et al.
Publicado: (2023)

Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance
por: Feng, Wei, et al.
Publicado: (2023)

Machine learning in the estimation of CRISPR-Cas9 cleavage sites for plant system
por: Das, Jutan, et al.
Publicado: (2023)

piCRISPR: Physically informed deep learning models for CRISPR/Cas9 off-target cleavage prediction
por: Störtz, Florian, et al.
Publicado: (2023)

Heterolytic Cleavage of Dihydrogen by an Iron(II) PNP Pincer Complex via Metal–Ligand Cooperation
por: Bichler, Bernhard, et al.
Publicado: (2013)

RNA-activated DNA cleavage by the Type III-B CRISPR–Cas effector complex
por: Estrella, Michael A., et al.
Publicado: (2016)

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
por: Ramanan, Vyas, et al.
Publicado: (2015)

Biased and Unbiased Methods for the Detection of Off-Target Cleavage by CRISPR/Cas9: An Overview
por: Martin, Francisco, et al.
Publicado: (2016)

Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
por: Zhang, Bo, et al.
Publicado: (2021)

CRISPR–Cas12a-mediated DNA clamping triggers target-strand cleavage
por: Naqvi, Mohsin M., et al.
Publicado: (2022)

BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
por: Zarate, Oscar A., et al.
Publicado: (2022)

GeneTEFlow: A Nextflow-based pipeline for analysing gene and transposable elements expression from RNA-Seq data
por: Liu, Xiaochuan, et al.
Publicado: (2020)

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
por: Zhang, Yilan, et al.
Publicado: (2014)

Target RNA capture and cleavage by the Cmr type III-B CRISPR–Cas effector complex
por: Hale, Caryn R., et al.
Publicado: (2014)

GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
por: Zhang, Bo, et al.
Publicado: (2019)

Permanent Inactivation of HBV Genomes by CRISPR/Cas9-Mediated Non-cleavage Base Editing
por: Yang, Yu-Chan, et al.
Publicado: (2020)

Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR–Cas12f nuclease
por: Xiao, Renjian, et al.
Publicado: (2021)

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage
por: Cheng, Liangfen, et al.
Publicado: (2022)

Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
por: Rossetti, Marianna, et al.
Publicado: (2022)

Structural insights into target DNA recognition and cleavage by the CRISPR-Cas12c1 system
por: Zhang, Bo, et al.
Publicado: (2022)

A CRISPR/Cas9 Cleavage System for Capturing Fungal Secondary Metabolite Gene Clusters
por: Xu, Xinran, et al.
Publicado: (2021)

CRISPR/Cas12a Collateral Cleavage Activity for Sensitive 3′–5′ Exonuclease Assay
por: Jeung, Jae Hoon, et al.
Publicado: (2023)

Lag-Screw Osteosynthesis in Thoracolumbar Pincer Fractures
por: Auerswald, Marc, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_q4bi5j8nju1v89kmmpbr6pi5hk