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Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species
Cytogenetic data for the genus Cichla Bloch et Schneider, 1801 are still very limited, with only four karyotype descriptions to date. The sum of the available cytogenetic information for Cichla species, points to a maintenance of the diploid number of 48 acrocentric chromosomes, considered a typical...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Pensoft Publishers
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515931/ https://www.ncbi.nlm.nih.gov/pubmed/33014295 http://dx.doi.org/10.3897/CompCytogen.v14i3.55279 |
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author | Quadros, Janice Ferreira, Alex M. V. Viana, Patrik F. Marajó, Leandro Oliveira, Ezequiel Ferreira, Efrem Feldberg, Eliana |
author_facet | Quadros, Janice Ferreira, Alex M. V. Viana, Patrik F. Marajó, Leandro Oliveira, Ezequiel Ferreira, Efrem Feldberg, Eliana |
author_sort | Quadros, Janice |
collection | PubMed |
description | Cytogenetic data for the genus Cichla Bloch et Schneider, 1801 are still very limited, with only four karyotype descriptions to date. The sum of the available cytogenetic information for Cichla species, points to a maintenance of the diploid number of 48 acrocentric chromosomes, considered a typical ancestral feature in cichlids. In the current study, we performed molecular and classical cytogenetic analyses of the karyotype organization of six species of Cichla, the earliest-diverging genus of Neotropical cichlids. We cytogenetically analysed Cichla kelberi Kullander et Ferreira, 2006, Cichla monoculus Agassiz, 1831, Cichla piquiti Kullander et Ferreira, 2006, Cichla temensis Humboldt, 1821, Cichla vazzoleri Kullander et Ferreira, 2006 and Cichla pinima Kullander et Ferreira, 2006, including three individuals that showed mixed morphological characteristics, likely from different species, suggesting they were hybrid individuals. All individuals analysed showed 2n = 48 acrocentric chromosomes, with centromeric heterochromatic blocks on all chromosomes and a terminal heterochromatic region on the q arm of the 2(nd) pair. Mapping 18S rDNA gave hybridization signals, correlated with the nucleolus organizer regions, on the 2(nd) pair for all analyzed individuals. However, we found distinct patterns for 5S rDNA: interstitially at the proximal position on 6(th) pair of four species (C. kelberi, C. pinima, C. piquiti and C. vazzoleri), and on the distal of the 4(th) pair in two (C. monoculus and C. temensis). Accordingly, we present here new data for the genus and discuss the evolutionary trends in the karyotype of this group of fish. In addition, we provide data that supports the occurrence of hybrid individuals in the Uatumã River region, mainly based on 5S rDNA mapping. |
format | Online Article Text |
id | pubmed-7515931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-75159312020-10-02 Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species Quadros, Janice Ferreira, Alex M. V. Viana, Patrik F. Marajó, Leandro Oliveira, Ezequiel Ferreira, Efrem Feldberg, Eliana Comp Cytogenet Research Article Cytogenetic data for the genus Cichla Bloch et Schneider, 1801 are still very limited, with only four karyotype descriptions to date. The sum of the available cytogenetic information for Cichla species, points to a maintenance of the diploid number of 48 acrocentric chromosomes, considered a typical ancestral feature in cichlids. In the current study, we performed molecular and classical cytogenetic analyses of the karyotype organization of six species of Cichla, the earliest-diverging genus of Neotropical cichlids. We cytogenetically analysed Cichla kelberi Kullander et Ferreira, 2006, Cichla monoculus Agassiz, 1831, Cichla piquiti Kullander et Ferreira, 2006, Cichla temensis Humboldt, 1821, Cichla vazzoleri Kullander et Ferreira, 2006 and Cichla pinima Kullander et Ferreira, 2006, including three individuals that showed mixed morphological characteristics, likely from different species, suggesting they were hybrid individuals. All individuals analysed showed 2n = 48 acrocentric chromosomes, with centromeric heterochromatic blocks on all chromosomes and a terminal heterochromatic region on the q arm of the 2(nd) pair. Mapping 18S rDNA gave hybridization signals, correlated with the nucleolus organizer regions, on the 2(nd) pair for all analyzed individuals. However, we found distinct patterns for 5S rDNA: interstitially at the proximal position on 6(th) pair of four species (C. kelberi, C. pinima, C. piquiti and C. vazzoleri), and on the distal of the 4(th) pair in two (C. monoculus and C. temensis). Accordingly, we present here new data for the genus and discuss the evolutionary trends in the karyotype of this group of fish. In addition, we provide data that supports the occurrence of hybrid individuals in the Uatumã River region, mainly based on 5S rDNA mapping. Pensoft Publishers 2020-09-17 /pmc/articles/PMC7515931/ /pubmed/33014295 http://dx.doi.org/10.3897/CompCytogen.v14i3.55279 Text en Janice Quadros, Alex M. V. Ferreira, Patrik F. Viana, Leandro Marajó, Ezequiel Oliveira, Efrem Ferreira, Eliana Feldberg http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Quadros, Janice Ferreira, Alex M. V. Viana, Patrik F. Marajó, Leandro Oliveira, Ezequiel Ferreira, Efrem Feldberg, Eliana Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title | Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title_full | Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title_fullStr | Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title_full_unstemmed | Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title_short | Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
title_sort | comparative cytogenetic of six species of amazonian peacock bass (cichla, cichlinae): intrachromosomal variations and genetic introgression among sympatric species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515931/ https://www.ncbi.nlm.nih.gov/pubmed/33014295 http://dx.doi.org/10.3897/CompCytogen.v14i3.55279 |
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