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MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease

Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer’s disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic ther...

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Autores principales: De Felice, Bruna, Montanino, Concetta, Oliva, Mariano, Bonavita, Simona, Di Onofrio, Valeria, Coppola, Cinzia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515963/
https://www.ncbi.nlm.nih.gov/pubmed/32737762
http://dx.doi.org/10.1007/s12035-020-02029-7
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author De Felice, Bruna
Montanino, Concetta
Oliva, Mariano
Bonavita, Simona
Di Onofrio, Valeria
Coppola, Cinzia
author_facet De Felice, Bruna
Montanino, Concetta
Oliva, Mariano
Bonavita, Simona
Di Onofrio, Valeria
Coppola, Cinzia
author_sort De Felice, Bruna
collection PubMed
description Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer’s disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In this study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD.
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spelling pubmed-75159632020-10-07 MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease De Felice, Bruna Montanino, Concetta Oliva, Mariano Bonavita, Simona Di Onofrio, Valeria Coppola, Cinzia Mol Neurobiol Article Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer’s disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In this study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD. Springer US 2020-07-31 2020 /pmc/articles/PMC7515963/ /pubmed/32737762 http://dx.doi.org/10.1007/s12035-020-02029-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
De Felice, Bruna
Montanino, Concetta
Oliva, Mariano
Bonavita, Simona
Di Onofrio, Valeria
Coppola, Cinzia
MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title_full MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title_fullStr MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title_full_unstemmed MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title_short MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
title_sort microrna expression signature in mild cognitive impairment due to alzheimer’s disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7515963/
https://www.ncbi.nlm.nih.gov/pubmed/32737762
http://dx.doi.org/10.1007/s12035-020-02029-7
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