Cargando…

German-Wide Interlaboratory Study Compares Consistency, Accuracy and Reproducibility of Whole-Genome Short Read Sequencing

We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria. Participants were asked to sequence six DNA samples of t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Uelze, Laura, Borowiak, Maria, Bönn, Markus, Brinks, Erik, Deneke, Carlus, Hankeln, Thomas, Kleta, Sylvia, Murr, Larissa, Stingl, Kerstin, Szabo, Kathrin, Tausch, Simon H., Wöhlke, Anne, Malorny, Burkhard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516015/ https://www.ncbi.nlm.nih.gov/pubmed/33013811 http://dx.doi.org/10.3389/fmicb.2020.573972

Ejemplares similares

First complete genome sequence and comparative analysis of Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) indicates host adaptation traits to sheep
por: Uelze, Laura, et al.
Publicado: (2019)

Performance and Accuracy of Four Open-Source Tools for In Silico Serotyping of Salmonella spp. Based on Whole-Genome Short-Read Sequencing Data
por: Uelze, Laura, et al.
Publicado: (2020)

Species-Specific Quality Control, Assembly and Contamination Detection in Microbial Isolate Sequences with AQUAMIS
por: Deneke, Carlus, et al.
Publicado: (2021)

Comparative genomics of Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) reveals lineage-specific host adaptation of ST432
por: Uelze, Laura, et al.
Publicado: (2021)

Decentralized Investigation of Bacterial Outbreaks Based on Hashed cgMLST
por: Deneke, Carlus, et al.
Publicado: (2021)

Typing methods based on whole genome sequencing data
por: Uelze, Laura, et al.
Publicado: (2020)

What WGS Reveals about Salmonella enterica subsp. enterica in Wildlife in Germany
por: Uelze, Laura, et al.
Publicado: (2021)

Complete Genome Sequence of Salmonella enterica subsp. diarizonae Serovar 61:k:1,5,(7) Strain 14-SA00836-0, Isolated from Human Urine
por: Uelze, Laura, et al.
Publicado: (2020)

Development of a Novel mcr-6 to mcr-9 Multiplex PCR and Assessment of mcr-1 to mcr-9 Occurrence in Colistin-Resistant Salmonella enterica Isolates From Environment, Feed, Animals and Food (2011–2018) in Germany
por: Borowiak, Maria, et al.
Publicado: (2020)

Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential
por: Golz, Julia C., et al.
Publicado: (2020)

Toward an Integrated Genome-Based Surveillance of Salmonella enterica in Germany
por: Uelze, Laura, et al.
Publicado: (2021)

Outcome of Different Sequencing and Assembly Approaches on the Detection of Plasmids and Localization of Antimicrobial Resistance Genes in Commensal Escherichia coli
por: Juraschek, Katharina, et al.
Publicado: (2021)

Fishing in the Soup – Pathogen Detection in Food Safety Using Metabarcoding and Metagenomic Sequencing
por: Grützke, Josephine, et al.
Publicado: (2019)

The bla(NDM-1)-Carrying IncA/C(2) Plasmid Underlies Structural Alterations and Cointegrate Formation In Vivo
por: Hadziabdic, Sead, et al.
Publicado: (2019)

Genetic characterization of a multidrug-resistant Salmonella enterica serovar Agona isolated from a dietary supplement in Germany
por: Bartsch, Lee Julia, et al.
Publicado: (2023)

Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017
por: Juraschek, Katharina, et al.
Publicado: (2021)

Genomic Insights into the Mobilome and Resistome of Sentinel Microorganisms Originating from Farms of Two Different Swine Production Systems
por: Mencía-Ares, Oscar, et al.
Publicado: (2022)

Translatability of WGS typing results can simplify data exchange for surveillance and control of Listeria monocytogenes
por: Lüth, Stefanie, et al.
Publicado: (2020)

Interlaboratory Reproducibility of Standardized Hemagglutination Inhibition Assays
por: Bibby, David C., et al.
Publicado: (2022)

Clonal relation between Salmonella enterica subspecies enterica serovar Dublin strains of bovine and food origin in Germany
por: Linde, Jörg, et al.
Publicado: (2023)

Robust interlaboratory reproducibility of a gene expression signature measurement consistent with the needs of a new generation of diagnostic tools
por: Ach, Robert A, et al.
Publicado: (2007)

Genetic and Phenotypic Virulence Potential of Non-O1/Non-O139 Vibrio cholerae Isolated from German Retail Seafood
por: Zhang, Quantao, et al.
Publicado: (2023)

Complete Genome Sequence of Salmonella enterica subsp. enterica Serovar Paratyphi B Sequence Type 28 Harboring mcr-1
por: Borowiak, Maria, et al.
Publicado: (2017)

Complete Genome Sequence of an Aeromonas rivuli Strain Isolated from Ready-to-Eat Food
por: Schwartz, Keike, et al.
Publicado: (2022)

Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium Synechocystis sp. PCC 6803
por: Mager, Maurice, et al.
Publicado: (2023)

Complete Genome Sequence of a VIM-1-Producing Salmonella enterica subsp. enterica Serovar Infantis Isolate Derived from Minced Pork Meat
por: Borowiak, Maria, et al.
Publicado: (2018)

Complete and Circularized Genome Assembly of a Human Isolate of Vibrio navarrensis Biotype pommerensis with MiSeq and MinION Sequence Data
por: Schwartz, Keike, et al.
Publicado: (2021)

Standardisation of EGFR FISH in colorectal cancer: results of an international interlaboratory reproducibility ring study
por: Sartore-Bianchi, Andrea, et al.
Publicado: (2011)

Detection of VIM-1-Producing Enterobacter cloacae and Salmonella enterica Serovars Infantis and Goldcoast at a Breeding Pig Farm in Germany in 2017 and Their Molecular Relationship to Former VIM-1-Producing S. Infantis Isolates in German Livestock Production
por: Roschanski, Nicole, et al.
Publicado: (2019)

Complete Genome Sequence of a Salmonella enterica subsp. enterica Serovar Tennessee Strain from Tahini
por: Schlund, Olga, et al.
Publicado: (2022)

Complete Genome Sequence of Arcanobacterium sp. Strain 2701, Isolated from a Harbor Seal
por: Borowiak, Maria, et al.
Publicado: (2020)

A step forward for Shiga toxin-producing Escherichia coli identification and characterization in raw milk using long-read metagenomics
por: Jaudou, Sandra, et al.
Publicado: (2022)

Development of Micro-Electrode Array Based Tests for Neurotoxicity: Assessment of Interlaboratory Reproducibility with Neuroactive Chemicals
por: Novellino, A., et al.
Publicado: (2011)

Long-term repeatability and interlaboratory reproducibility of high-precision ID-TIMS U–Pb geochronology
por: Schaltegger, Urs, et al.
Publicado: (2021)

Direct identification and molecular characterization of zoonotic hazards in raw milk by metagenomics using Brucella as a model pathogen
por: Grützke, Josephine, et al.
Publicado: (2021)

Complete Genome Sequence of an Avian Native NDM-1-Producing Salmonella enterica subsp. enterica Serovar Corvallis Strain
por: Hadziabdic, Sead, et al.
Publicado: (2018)

Exploring Long-Read Metagenomics for Full Characterization of Shiga Toxin-Producing Escherichia coli in Presence of Commensal E. coli
por: Jaudou, Sandra, et al.
Publicado: (2023)

High Interlaboratory Reproducibility and Accuracy of Next-Generation-Sequencing-Based Bacterial Genotyping in a Ring Trial
por: Mellmann, Alexander, et al.
Publicado: (2017)

Travelling Wave Ion Mobility-Derived Collision Cross Section for Mycotoxins: Investigating Interlaboratory and Interplatform Reproducibility
por: Righetti, Laura, et al.
Publicado: (2020)

In Vivo Transfer and Microevolution of Avian Native IncA/C(2) bla(NDM-1)-Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study
por: Hadziabdic, Sead, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_f8ie3mcf3138fqe0dd7edscner