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Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus

Dendritic cells (DCs) are essential for successful embryo implantation. However, the properties of uterine DCs (uDCs) during the implantation period are not well characterized. In this study, we investigated the dynamic changes in the uDC phenotypes during the period between coitus and implantation....

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Autores principales: Yasuda, Ippei, Shima, Tomoko, Moriya, Taiki, Ikebuchi, Ryoyo, Kusumoto, Yutaka, Ushijima, Akemi, Nakashima, Akitoshi, Tomura, Michio, Saito, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516021/
https://www.ncbi.nlm.nih.gov/pubmed/33013926
http://dx.doi.org/10.3389/fimmu.2020.557720
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author Yasuda, Ippei
Shima, Tomoko
Moriya, Taiki
Ikebuchi, Ryoyo
Kusumoto, Yutaka
Ushijima, Akemi
Nakashima, Akitoshi
Tomura, Michio
Saito, Shigeru
author_facet Yasuda, Ippei
Shima, Tomoko
Moriya, Taiki
Ikebuchi, Ryoyo
Kusumoto, Yutaka
Ushijima, Akemi
Nakashima, Akitoshi
Tomura, Michio
Saito, Shigeru
author_sort Yasuda, Ippei
collection PubMed
description Dendritic cells (DCs) are essential for successful embryo implantation. However, the properties of uterine DCs (uDCs) during the implantation period are not well characterized. In this study, we investigated the dynamic changes in the uDC phenotypes during the period between coitus and implantation. In virgin mice, we evaluated the expressions of CD103 and XCR1, this is the first report to demonstrate uDCs expressing CD103 in XCR1(+)cDC1s and XCR1(+)cDC2s. On day 0.5 post coitus (pc), the number of uterine CD11c(+)CD103(–)MHC classII(high)CD86(high)–mature DCs rapidly increased and then decreased to non-pregnancy levels on days 1.5 and 2.5 pc. On day 3.5 pc just before implantation, the number of CD11c(+)CD103(+)MHC class II(dim)CD86(dim)–immature DCs increased in the uterus. The increase in mature uDCs on day 1.5 pc was observed in both allogeneic- and syngeneic mating, suggesting that sexual intercourse, or semen, play a role in this process. Meanwhile, the increase in immature uDCs on day 3.5 pc was only observed in allogeneic mating, suggesting that allo-antigens in the semen contribute to this process. Next, to understand the turnover and migration of uDCs, we monitored DC movement in the uterus and uterine draining lymph nodes (dLNs) using photoconvertible protein Kikume Green Red (KikGR) mice. On day 0.5 pc, uDCs were composed of equal numbers of remaining DCs and migratory DCs. However, on day 3.5 pc, uDCs were primarily composed of migratory DCs, suggesting that most of the uDCs migrate from the periphery just before implantation. Finally, we studied the expression of PD-L2—which induces immunoregulation—on DCs. On day 3.5 pc, PD-L2 was expressed on CD103(+)-mature and CD103(–)-mature DCs in the uterus. However, PD-L2 expression on CD103(–)-immature DCs and CD103(+)-immature DCs was very low. Furthermore, both remaining and migratory DCs in the uterus and uterus-derived-DCs in the dLNs on day 3.5 pc highly expressed PD-L2 on their surface. Therefore, our study findings provide a better understanding of the dynamic changes occurring in uterine DCs and dLNs in preparation for implantation following allogeneic- and syngeneic mating.
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spelling pubmed-75160212020-10-02 Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus Yasuda, Ippei Shima, Tomoko Moriya, Taiki Ikebuchi, Ryoyo Kusumoto, Yutaka Ushijima, Akemi Nakashima, Akitoshi Tomura, Michio Saito, Shigeru Front Immunol Immunology Dendritic cells (DCs) are essential for successful embryo implantation. However, the properties of uterine DCs (uDCs) during the implantation period are not well characterized. In this study, we investigated the dynamic changes in the uDC phenotypes during the period between coitus and implantation. In virgin mice, we evaluated the expressions of CD103 and XCR1, this is the first report to demonstrate uDCs expressing CD103 in XCR1(+)cDC1s and XCR1(+)cDC2s. On day 0.5 post coitus (pc), the number of uterine CD11c(+)CD103(–)MHC classII(high)CD86(high)–mature DCs rapidly increased and then decreased to non-pregnancy levels on days 1.5 and 2.5 pc. On day 3.5 pc just before implantation, the number of CD11c(+)CD103(+)MHC class II(dim)CD86(dim)–immature DCs increased in the uterus. The increase in mature uDCs on day 1.5 pc was observed in both allogeneic- and syngeneic mating, suggesting that sexual intercourse, or semen, play a role in this process. Meanwhile, the increase in immature uDCs on day 3.5 pc was only observed in allogeneic mating, suggesting that allo-antigens in the semen contribute to this process. Next, to understand the turnover and migration of uDCs, we monitored DC movement in the uterus and uterine draining lymph nodes (dLNs) using photoconvertible protein Kikume Green Red (KikGR) mice. On day 0.5 pc, uDCs were composed of equal numbers of remaining DCs and migratory DCs. However, on day 3.5 pc, uDCs were primarily composed of migratory DCs, suggesting that most of the uDCs migrate from the periphery just before implantation. Finally, we studied the expression of PD-L2—which induces immunoregulation—on DCs. On day 3.5 pc, PD-L2 was expressed on CD103(+)-mature and CD103(–)-mature DCs in the uterus. However, PD-L2 expression on CD103(–)-immature DCs and CD103(+)-immature DCs was very low. Furthermore, both remaining and migratory DCs in the uterus and uterus-derived-DCs in the dLNs on day 3.5 pc highly expressed PD-L2 on their surface. Therefore, our study findings provide a better understanding of the dynamic changes occurring in uterine DCs and dLNs in preparation for implantation following allogeneic- and syngeneic mating. Frontiers Media S.A. 2020-09-11 /pmc/articles/PMC7516021/ /pubmed/33013926 http://dx.doi.org/10.3389/fimmu.2020.557720 Text en Copyright © 2020 Yasuda, Shima, Moriya, Ikebuchi, Kusumoto, Ushijima, Nakashima, Tomura and Saito. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Yasuda, Ippei
Shima, Tomoko
Moriya, Taiki
Ikebuchi, Ryoyo
Kusumoto, Yutaka
Ushijima, Akemi
Nakashima, Akitoshi
Tomura, Michio
Saito, Shigeru
Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title_full Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title_fullStr Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title_full_unstemmed Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title_short Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus
title_sort dynamic changes in the phenotype of dendritic cells in the uterus and uterine draining lymph nodes after coitus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516021/
https://www.ncbi.nlm.nih.gov/pubmed/33013926
http://dx.doi.org/10.3389/fimmu.2020.557720
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