Cargando…

Systematic Quantification of Synapses in Primary Neuronal Culture

Most neurological disorders display impaired synaptic connectivity. Hence, modulation of synapse formation may have therapeutic relevance. However, the high density and small size of synapses complicate their quantification. To improve synapse-oriented screens, we analyzed the labeling performance o...

Descripción completa

Detalles Bibliográficos
Autores principales:	Verstraelen, Peter, Garcia-Diaz Barriga, Gerardo, Verschuuren, Marlies, Asselbergh, Bob, Nuydens, Rony, Larsen, Peter H., Timmermans, Jean-Pierre, De Vos, Winnok H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516133/ https://www.ncbi.nlm.nih.gov/pubmed/33083769 http://dx.doi.org/10.1016/j.isci.2020.101542

Ejemplares similares

Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture
por: Verstraelen, Peter, et al.
Publicado: (2018)

High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity
por: Verschuuren, Marlies, et al.
Publicado: (2019)

Dysregulation of Microtubule Stability Impairs Morphofunctional Connectivity in Primary Neuronal Networks
por: Verstraelen, Peter, et al.
Publicado: (2017)

Sustained synchronized neuronal network activity in a human astrocyte co-culture system
por: Kuijlaars, Jacobine, et al.
Publicado: (2016)

Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation
por: Xiong, Ranhua, et al.
Publicado: (2018)

Induced pluripotent stem cell-derived motor neurons of CMT type 2 patients reveal progressive mitochondrial dysfunction
por: Van Lent, Jonas, et al.
Publicado: (2021)

Accurate Detection of Dysmorphic Nuclei Using Dynamic Programming and Supervised Classification
por: Verschuuren, Marlies, et al.
Publicado: (2017)

Pharmacological evaluation of rat dorsal root ganglion neurons as an in vitro model for diabetic neuropathy
por: Peeraer, Eve, et al.
Publicado: (2011)

Learning-guided automatic three dimensional synapse quantification for drosophila neurons
por: Sanders, Jonathan, et al.
Publicado: (2015)

Mutant HSPB8 causes motor neuron-specific neurite degeneration
por: Irobi, Joy, et al.
Publicado: (2010)

Prediction of biological age by morphological staging of sarcopenia in Caenorhabditis elegans
por: Dhondt, Ineke, et al.
Publicado: (2021)

Opioid-induced proliferation of vascular endothelial cells
por: Leo, Sandra, et al.
Publicado: (2009)

Transient nuclear lamin A/C accretion aids in recovery from vapor nanobubble-induced permeabilisation of the plasma membrane
por: Houthaeve, Gaëlle, et al.
Publicado: (2022)

DIFFERENTIATION OF NEURONAL TYPES AND SYNAPSES IN MYELINATING CULTURES OF MOUSE CEREBELLUM
por: Wolf, Merrill K.
Publicado: (1964)

Charcot–Marie–Tooth causing HSPB1 mutations increase Cdk5-mediated phosphorylation of neurofilaments
por: Holmgren, Anne, et al.
Publicado: (2013)

Effects of metformin on tumor hypoxia and radiotherapy efficacy: a [(18)F]HX4 PET imaging study in colorectal cancer xenografts
por: De Bruycker, Sven, et al.
Publicado: (2019)

Asymmetric N-Cadherin Expression Results in Synapse Dysfunction, Synapse Elimination, and Axon Retraction in Cultured Mouse Neurons
por: Pielarski, Kim N., et al.
Publicado: (2013)

Effects of Trace Metal Profiles Characteristic for Autism on Synapses in Cultured Neurons
por: Hagmeyer, Simone, et al.
Publicado: (2015)

Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures
por: Green, Matthew V., et al.
Publicado: (2019)

Neuroproteomics of the Synapse: Subcellular Quantification of Protein Networks and Signaling Dynamics
por: van Gelder, Charlotte A.G.H., et al.
Publicado: (2021)

A novel unbiased counting method for the quantification of synapses in the mouse brain
por: Reichmann, Florian, et al.
Publicado: (2015)

Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies
por: Haidar, Mansour, et al.
Publicado: (2019)

Altered basal forebrain function during whole-brain network activity at pre- and early-plaque stages of Alzheimer’s disease in TgF344-AD rats
por: van den Berg, Monica, et al.
Publicado: (2022)

Neuronal epigenetics and the aging synapse
por: Azpurua, Jorge, et al.
Publicado: (2015)

Brain Mapping and Synapse Quantification In vivo: It's Time to Imaging
por: Acebes, Angel
Publicado: (2017)

Auranofin Synergizes with the PARP Inhibitor Olaparib to Induce ROS-Mediated Cell Death in Mutant p53 Cancers
por: Freire Boullosa, Laurie, et al.
Publicado: (2023)

Lytic granule exocytosis at immune synapses: lessons from neuronal synapses
por: Chang, Hsin-Fang, et al.
Publicado: (2023)

Synapse fits neuron: joint reduction by model inversion
por: van der Scheer, H. T., et al.
Publicado: (2017)

Coexistence of glutamatergic spine synapses and shaft synapses in substantia nigra dopamine neurons
por: Jang, Miae, et al.
Publicado: (2015)

Methods for neuronal guiding and synapse formation
por: Banciu, DD, et al.
Publicado: (2012)

MoniTORing neuronal excitability at the synapse
por: Kepert, Inge, et al.
Publicado: (2013)

Neuroligins/LRRTMs prevent activity- and Ca(2+)/calmodulin-dependent synapse elimination in cultured neurons
por: Ko, Jaewon, et al.
Publicado: (2011)

Sphingosine 1-Phoshpate Receptors are Located in Synapses and Control Spontaneous Activity of Mouse Neurons in Culture
por: Skoug, Cecilia, et al.
Publicado: (2022)

CNS Neurons Deposit Laminin α5 to Stabilize Synapses
por: Omar, Mitchell H., et al.
Publicado: (2017)

Quantification of the density of cooperative neighboring synapses required to evoke endocannabinoid signaling()
por: Pachoud, B., et al.
Publicado: (2014)

Dendritic and Axonal Propagation Delays May Shape Neuronal Networks With Plastic Synapses
por: Madadi Asl, Mojtaba, et al.
Publicado: (2018)

Neuronal activity moves protein palmitoylation into the synapse
por: Dalva, Matthew B.
Publicado: (2009)

Activity-dependent expression of Channelrhodopsin at neuronal synapses
por: Gobbo, Francesco, et al.
Publicado: (2017)

ELKS1 helps neuronal synapses diversify
por: Short, Ben
Publicado: (2017)

Nanomachinery Organizing Release at Neuronal and Ribbon Synapses
por: Chakrabarti, Rituparna, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_kss7rnqqarg6anmq9o2q7dcdgc