Cargando…
The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue
BACKGROUND: Lung cancer is a leading cause of cancer morbidity and mortality worldwide. Several studies have suggested that Human papillomavirus (HPV) infection is an important risk factor in the development of lung cancer. In this study, we aim to address the role of HPV in the development of lung...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517685/ https://www.ncbi.nlm.nih.gov/pubmed/32972386 http://dx.doi.org/10.1186/s12885-020-07428-6 |
_version_ | 1783587273128804352 |
---|---|
author | Rezaei, Marzieh Mostafaei, Shayan Aghaei, Amir Hosseini, Nayyerehalsadat Darabi, Hassan Nouri, Majid Etemadi, Ashkan Neill, Andrew O’. Nahand, Javid Sadri Mirzaei, Hamed Donnelly, Seamas C. Doroudian, Mohammad Moghoofei, Mohsen |
author_facet | Rezaei, Marzieh Mostafaei, Shayan Aghaei, Amir Hosseini, Nayyerehalsadat Darabi, Hassan Nouri, Majid Etemadi, Ashkan Neill, Andrew O’. Nahand, Javid Sadri Mirzaei, Hamed Donnelly, Seamas C. Doroudian, Mohammad Moghoofei, Mohsen |
author_sort | Rezaei, Marzieh |
collection | PubMed |
description | BACKGROUND: Lung cancer is a leading cause of cancer morbidity and mortality worldwide. Several studies have suggested that Human papillomavirus (HPV) infection is an important risk factor in the development of lung cancer. In this study, we aim to address the role of HPV in the development of lung cancer mechanistically by examining the induction of inflammation and epithelial-mesenchymal transition (EMT) by this virus. METHODS: In this case-control study, tissue samples were collected from 102 cases with lung cancer and 48 controls. We examined the presence of HPV DNA and also the viral genotype in positive samples. We also examined the expression of viral genes (E2, E6 and E7), anti-carcinogenic genes (p53, retinoblastoma (RB)), and inflammatory cytokines in HPV positive cases. RESULTS: HPV DNA was detected in 52.9% (54/102) of the case samples and in 25% (12/48) of controls. A significant association was observed between a HPV positive status and lung cancer (OR = 3.37, 95% C.I = 1.58–7.22, P = 0.001). The most prevalent virus genotype in the patients was type 16 (38.8%). The expression of p53 and RB were decreased while and inflammatory cytokines were increased in HPV-positive lung cancer and HPV-positive control tissues compared to HPV-negative lung cancer and HPV-negative control tissues. Also, the expression level of E-cad and PTPN-13 genes were decreased in HPV- positive samples while the expression level of SLUG, TWIST and N-cad was increased in HPV-positive samples compared to negative samples. CONCLUSION: Our study suggests that HPV infection drives the induction of inflammation and EMT which may promote in the development of lung cancer. |
format | Online Article Text |
id | pubmed-7517685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75176852020-09-25 The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue Rezaei, Marzieh Mostafaei, Shayan Aghaei, Amir Hosseini, Nayyerehalsadat Darabi, Hassan Nouri, Majid Etemadi, Ashkan Neill, Andrew O’. Nahand, Javid Sadri Mirzaei, Hamed Donnelly, Seamas C. Doroudian, Mohammad Moghoofei, Mohsen BMC Cancer Research Article BACKGROUND: Lung cancer is a leading cause of cancer morbidity and mortality worldwide. Several studies have suggested that Human papillomavirus (HPV) infection is an important risk factor in the development of lung cancer. In this study, we aim to address the role of HPV in the development of lung cancer mechanistically by examining the induction of inflammation and epithelial-mesenchymal transition (EMT) by this virus. METHODS: In this case-control study, tissue samples were collected from 102 cases with lung cancer and 48 controls. We examined the presence of HPV DNA and also the viral genotype in positive samples. We also examined the expression of viral genes (E2, E6 and E7), anti-carcinogenic genes (p53, retinoblastoma (RB)), and inflammatory cytokines in HPV positive cases. RESULTS: HPV DNA was detected in 52.9% (54/102) of the case samples and in 25% (12/48) of controls. A significant association was observed between a HPV positive status and lung cancer (OR = 3.37, 95% C.I = 1.58–7.22, P = 0.001). The most prevalent virus genotype in the patients was type 16 (38.8%). The expression of p53 and RB were decreased while and inflammatory cytokines were increased in HPV-positive lung cancer and HPV-positive control tissues compared to HPV-negative lung cancer and HPV-negative control tissues. Also, the expression level of E-cad and PTPN-13 genes were decreased in HPV- positive samples while the expression level of SLUG, TWIST and N-cad was increased in HPV-positive samples compared to negative samples. CONCLUSION: Our study suggests that HPV infection drives the induction of inflammation and EMT which may promote in the development of lung cancer. BioMed Central 2020-09-24 /pmc/articles/PMC7517685/ /pubmed/32972386 http://dx.doi.org/10.1186/s12885-020-07428-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Rezaei, Marzieh Mostafaei, Shayan Aghaei, Amir Hosseini, Nayyerehalsadat Darabi, Hassan Nouri, Majid Etemadi, Ashkan Neill, Andrew O’. Nahand, Javid Sadri Mirzaei, Hamed Donnelly, Seamas C. Doroudian, Mohammad Moghoofei, Mohsen The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title | The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title_full | The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title_fullStr | The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title_full_unstemmed | The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title_short | The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue |
title_sort | association between hpv gene expression, inflammatory agents and cellular genes involved in emt in lung cancer tissue |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517685/ https://www.ncbi.nlm.nih.gov/pubmed/32972386 http://dx.doi.org/10.1186/s12885-020-07428-6 |
work_keys_str_mv | AT rezaeimarzieh theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT mostafaeishayan theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT aghaeiamir theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT hosseininayyerehalsadat theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT darabihassan theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT nourimajid theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT etemadiashkan theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT neillandrewo theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT nahandjavidsadri theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT mirzaeihamed theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT donnellyseamasc theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT doroudianmohammad theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT moghoofeimohsen theassociationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT rezaeimarzieh associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT mostafaeishayan associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT aghaeiamir associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT hosseininayyerehalsadat associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT darabihassan associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT nourimajid associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT etemadiashkan associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT neillandrewo associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT nahandjavidsadri associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT mirzaeihamed associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT donnellyseamasc associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT doroudianmohammad associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue AT moghoofeimohsen associationbetweenhpvgeneexpressioninflammatoryagentsandcellulargenesinvolvedinemtinlungcancertissue |