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Rapid fabrication of sieved microwells and cross-flow microparticle trapping
The use of microwells is popular for a wide range of applications due to its’ simplicity. However, the seeding of conventional microwells, which are closed at the bottom, is restricted to gravitational sedimentation for cell or particle deposition and therefore require lengthy settling times to maxi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518267/ https://www.ncbi.nlm.nih.gov/pubmed/32973304 http://dx.doi.org/10.1038/s41598-020-72700-5 |
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author | Romita, Lauren Thompson, Shyan Hwang, Dae Kun |
author_facet | Romita, Lauren Thompson, Shyan Hwang, Dae Kun |
author_sort | Romita, Lauren |
collection | PubMed |
description | The use of microwells is popular for a wide range of applications due to its’ simplicity. However, the seeding of conventional microwells, which are closed at the bottom, is restricted to gravitational sedimentation for cell or particle deposition and therefore require lengthy settling times to maximize well occupancy. The addition of microfluidics to the capture process has accelerated cell or particle dispersion and improved capture ability but is mostly limited to gravitationally-driven settling for capture into the wells. An alternative approach to conventional closed-microwells, sieved microwells supersedes reliance on gravity by using hydrodynamic forces through the open pores at the bottom of the microwells to draw targets into the wells. We have developed a rapid fabrication method, based on flow lithography techniques, which allows us to easily customize the mesh pore sizes in a simple two-step process. Finally, by combining this microwell design with cross-flow trapping in a microfluidic two-layered channel, we achieve an 88 ± 6% well occupancy in under 10 s. |
format | Online Article Text |
id | pubmed-7518267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75182672020-09-29 Rapid fabrication of sieved microwells and cross-flow microparticle trapping Romita, Lauren Thompson, Shyan Hwang, Dae Kun Sci Rep Article The use of microwells is popular for a wide range of applications due to its’ simplicity. However, the seeding of conventional microwells, which are closed at the bottom, is restricted to gravitational sedimentation for cell or particle deposition and therefore require lengthy settling times to maximize well occupancy. The addition of microfluidics to the capture process has accelerated cell or particle dispersion and improved capture ability but is mostly limited to gravitationally-driven settling for capture into the wells. An alternative approach to conventional closed-microwells, sieved microwells supersedes reliance on gravity by using hydrodynamic forces through the open pores at the bottom of the microwells to draw targets into the wells. We have developed a rapid fabrication method, based on flow lithography techniques, which allows us to easily customize the mesh pore sizes in a simple two-step process. Finally, by combining this microwell design with cross-flow trapping in a microfluidic two-layered channel, we achieve an 88 ± 6% well occupancy in under 10 s. Nature Publishing Group UK 2020-09-24 /pmc/articles/PMC7518267/ /pubmed/32973304 http://dx.doi.org/10.1038/s41598-020-72700-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Romita, Lauren Thompson, Shyan Hwang, Dae Kun Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title | Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title_full | Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title_fullStr | Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title_full_unstemmed | Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title_short | Rapid fabrication of sieved microwells and cross-flow microparticle trapping |
title_sort | rapid fabrication of sieved microwells and cross-flow microparticle trapping |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518267/ https://www.ncbi.nlm.nih.gov/pubmed/32973304 http://dx.doi.org/10.1038/s41598-020-72700-5 |
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