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Determination of amyloid core regions of insulin analogues fibrils
A rapid-acting insulin lispro and long-acting insulin glargine are commonly used for the treatment of diabetes. Clinical cases have described the formation of injectable amyloidosis with these insulin analogues, but their amyloid core regions of fibrils were unknown. To reveal these regions, we have...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518739/ https://www.ncbi.nlm.nih.gov/pubmed/32543316 http://dx.doi.org/10.1080/19336896.2020.1776062 |
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author | Surin, Alexey K. Grishin, Sergei Yu. Galzitskaya, Oxana V. |
author_facet | Surin, Alexey K. Grishin, Sergei Yu. Galzitskaya, Oxana V. |
author_sort | Surin, Alexey K. |
collection | PubMed |
description | A rapid-acting insulin lispro and long-acting insulin glargine are commonly used for the treatment of diabetes. Clinical cases have described the formation of injectable amyloidosis with these insulin analogues, but their amyloid core regions of fibrils were unknown. To reveal these regions, we have analysed the hydrolyzates of insulin fibrils and its analogues using high-performance liquid chromatography and mass spectrometry methods and found that insulin and its analogues have almost identical amyloid core regions that intersect with the predicted amyloidogenic regions. The obtained results can be used to create new insulin analogues with a low ability to form fibrils. ABBREVIATIONS: a.a., amino acid residues; HPLC-MS, high-performance liquid chromatography/mass spectrometry; m/z, mass-to-charge ratio; TEM, transmission electron microscopy. |
format | Online Article Text |
id | pubmed-7518739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-75187392020-10-01 Determination of amyloid core regions of insulin analogues fibrils Surin, Alexey K. Grishin, Sergei Yu. Galzitskaya, Oxana V. Prion Research Paper A rapid-acting insulin lispro and long-acting insulin glargine are commonly used for the treatment of diabetes. Clinical cases have described the formation of injectable amyloidosis with these insulin analogues, but their amyloid core regions of fibrils were unknown. To reveal these regions, we have analysed the hydrolyzates of insulin fibrils and its analogues using high-performance liquid chromatography and mass spectrometry methods and found that insulin and its analogues have almost identical amyloid core regions that intersect with the predicted amyloidogenic regions. The obtained results can be used to create new insulin analogues with a low ability to form fibrils. ABBREVIATIONS: a.a., amino acid residues; HPLC-MS, high-performance liquid chromatography/mass spectrometry; m/z, mass-to-charge ratio; TEM, transmission electron microscopy. Taylor & Francis 2020-06-16 /pmc/articles/PMC7518739/ /pubmed/32543316 http://dx.doi.org/10.1080/19336896.2020.1776062 Text en © 2020 Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Surin, Alexey K. Grishin, Sergei Yu. Galzitskaya, Oxana V. Determination of amyloid core regions of insulin analogues fibrils |
title | Determination of amyloid core regions of insulin analogues fibrils |
title_full | Determination of amyloid core regions of insulin analogues fibrils |
title_fullStr | Determination of amyloid core regions of insulin analogues fibrils |
title_full_unstemmed | Determination of amyloid core regions of insulin analogues fibrils |
title_short | Determination of amyloid core regions of insulin analogues fibrils |
title_sort | determination of amyloid core regions of insulin analogues fibrils |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518739/ https://www.ncbi.nlm.nih.gov/pubmed/32543316 http://dx.doi.org/10.1080/19336896.2020.1776062 |
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