Cargando…

Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression

Growing evidence indicated that single nucleotide polymorphisms (SNPs) in the apolipoprotein E (APOE) gene are related to increase the risk of many inflammatory-related diseases. However, few genetic studies have associated the APOE gene polymorphism with sepsis. This study was to investigate the cl...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Yiming, Zhao, Tian, Zhang, Wenying, He, Junbing, Lu, Furong, Cai, Yujie, Lai, Zhipeng, Wei, Ning, Liang, Chunmei, Liu, Lizhen, Hong, Yuan, Cheng, Xiaohong, Li, Jia, Tang, Pei, Fan, Weihao, Ou, Mingqian, Yang, Jingqi, Liu, Yansong, Cui, Lili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519096/
https://www.ncbi.nlm.nih.gov/pubmed/32978453
http://dx.doi.org/10.1038/s41598-020-72616-0
_version_ 1783587510872440832
author Shao, Yiming
Zhao, Tian
Zhang, Wenying
He, Junbing
Lu, Furong
Cai, Yujie
Lai, Zhipeng
Wei, Ning
Liang, Chunmei
Liu, Lizhen
Hong, Yuan
Cheng, Xiaohong
Li, Jia
Tang, Pei
Fan, Weihao
Ou, Mingqian
Yang, Jingqi
Liu, Yansong
Cui, Lili
author_facet Shao, Yiming
Zhao, Tian
Zhang, Wenying
He, Junbing
Lu, Furong
Cai, Yujie
Lai, Zhipeng
Wei, Ning
Liang, Chunmei
Liu, Lizhen
Hong, Yuan
Cheng, Xiaohong
Li, Jia
Tang, Pei
Fan, Weihao
Ou, Mingqian
Yang, Jingqi
Liu, Yansong
Cui, Lili
author_sort Shao, Yiming
collection PubMed
description Growing evidence indicated that single nucleotide polymorphisms (SNPs) in the apolipoprotein E (APOE) gene are related to increase the risk of many inflammatory-related diseases. However, few genetic studies have associated the APOE gene polymorphism with sepsis. This study was to investigate the clinical relevance of the APOE gene polymorphism in the onset and progression of sepsis. A multicenter case–control association study with a large sample size (601 septic patients and 699 healthy individuals) was conducted. Clinical data showed that the APOEε4 allele was overrepresented among all patients with septic shock (p = 0.031) compared with sepsis subtype, suggesting that APOEε4 allele may associated with increased susceptibility to the progression of sepsis. Moreover, the APOE mRNA levels decreased after lipopolysaccharide (LPS) stimulation in cells in culture. Then 21 healthy individuals to extract PBMC for genotype grouping (APOE4+ group 8; APOE4− group 13) was selected to evaluate the effect on APOE level, and results showed that the expression level of APOE in APOE4+ group and APOE4− group did not differ in mRNA levels after an LPS challenge, but the protein levels in APOE4+ group decreased slower than that in APOE4− group, and this process was accompanied by the upregulation of proinflammatory cytokines. These results provide evidence that the APOEε4 allele might be associated with the development of sepsis and a potential risk factor that can be used in the prognosis of sepsis.
format Online
Article
Text
id pubmed-7519096
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-75190962020-09-29 Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression Shao, Yiming Zhao, Tian Zhang, Wenying He, Junbing Lu, Furong Cai, Yujie Lai, Zhipeng Wei, Ning Liang, Chunmei Liu, Lizhen Hong, Yuan Cheng, Xiaohong Li, Jia Tang, Pei Fan, Weihao Ou, Mingqian Yang, Jingqi Liu, Yansong Cui, Lili Sci Rep Article Growing evidence indicated that single nucleotide polymorphisms (SNPs) in the apolipoprotein E (APOE) gene are related to increase the risk of many inflammatory-related diseases. However, few genetic studies have associated the APOE gene polymorphism with sepsis. This study was to investigate the clinical relevance of the APOE gene polymorphism in the onset and progression of sepsis. A multicenter case–control association study with a large sample size (601 septic patients and 699 healthy individuals) was conducted. Clinical data showed that the APOEε4 allele was overrepresented among all patients with septic shock (p = 0.031) compared with sepsis subtype, suggesting that APOEε4 allele may associated with increased susceptibility to the progression of sepsis. Moreover, the APOE mRNA levels decreased after lipopolysaccharide (LPS) stimulation in cells in culture. Then 21 healthy individuals to extract PBMC for genotype grouping (APOE4+ group 8; APOE4− group 13) was selected to evaluate the effect on APOE level, and results showed that the expression level of APOE in APOE4+ group and APOE4− group did not differ in mRNA levels after an LPS challenge, but the protein levels in APOE4+ group decreased slower than that in APOE4− group, and this process was accompanied by the upregulation of proinflammatory cytokines. These results provide evidence that the APOEε4 allele might be associated with the development of sepsis and a potential risk factor that can be used in the prognosis of sepsis. Nature Publishing Group UK 2020-09-25 /pmc/articles/PMC7519096/ /pubmed/32978453 http://dx.doi.org/10.1038/s41598-020-72616-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shao, Yiming
Zhao, Tian
Zhang, Wenying
He, Junbing
Lu, Furong
Cai, Yujie
Lai, Zhipeng
Wei, Ning
Liang, Chunmei
Liu, Lizhen
Hong, Yuan
Cheng, Xiaohong
Li, Jia
Tang, Pei
Fan, Weihao
Ou, Mingqian
Yang, Jingqi
Liu, Yansong
Cui, Lili
Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title_full Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title_fullStr Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title_full_unstemmed Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title_short Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression
title_sort presence of the apolipoprotein e-ε4 allele is associated with an increased risk of sepsis progression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519096/
https://www.ncbi.nlm.nih.gov/pubmed/32978453
http://dx.doi.org/10.1038/s41598-020-72616-0
work_keys_str_mv AT shaoyiming presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT zhaotian presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT zhangwenying presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT hejunbing presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT lufurong presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT caiyujie presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT laizhipeng presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT weining presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT liangchunmei presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT liulizhen presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT hongyuan presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT chengxiaohong presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT lijia presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT tangpei presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT fanweihao presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT oumingqian presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT yangjingqi presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT liuyansong presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression
AT cuilili presenceoftheapolipoproteinee4alleleisassociatedwithanincreasedriskofsepsisprogression