CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma

PURPOSE: This study aimed to elucidate the biological function and upstream regulatory mechanism of CELSR1 in glioma. MATERIALS AND METHODS: We evaluated the expression of CELSR1 in glioma by TCGA_GEPIA tool, RT-qPCR, and Western blot assays. CCK-8, wound healing, and transwell invasion assays were,...

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Autores principales: Wang, Guang, Li, Yong, Zhang, Dongxia, Zhao, Songtao, Zhang, Qiong, Luo, Chao, Sun, Xiaochuan, Zhang, Bingqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520142/
https://www.ncbi.nlm.nih.gov/pubmed/33061581
http://dx.doi.org/10.2147/CMAR.S258835
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author Wang, Guang
Li, Yong
Zhang, Dongxia
Zhao, Songtao
Zhang, Qiong
Luo, Chao
Sun, Xiaochuan
Zhang, Bingqian
author_facet Wang, Guang
Li, Yong
Zhang, Dongxia
Zhao, Songtao
Zhang, Qiong
Luo, Chao
Sun, Xiaochuan
Zhang, Bingqian
author_sort Wang, Guang
collection PubMed
description PURPOSE: This study aimed to elucidate the biological function and upstream regulatory mechanism of CELSR1 in glioma. MATERIALS AND METHODS: We evaluated the expression of CELSR1 in glioma by TCGA_GEPIA tool, RT-qPCR, and Western blot assays. CCK-8, wound healing, and transwell invasion assays were, respectively, performed to detect the effect of CELSR1 on cell proliferation, migration, and invasion. The upstream regulatory miRNAs of CELSR1 were predicted by TargetScan and validated by luciferase activity reporter assay. RESULTS: CELSR1 is overexpressed in glioma (P<0.05). CELSR1 promoted glioma cell proliferation, migration and invasion (P<0.01). CELSR1 was a direct target of miR-199a-5p. miR199a-5p mimics significantly inhibited CELSR1 mRNA and protein expression (P<0.01). miR199a-5p mimics reversed the effects of CELSR1 on glioma cell behaviors (P<0.01). CONCLUSION: CELSR1 acts as an oncogene promoting glioma cell proliferation, migration, and invasion, which is regulated by miR199a-5p.
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spelling pubmed-75201422020-10-14 CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma Wang, Guang Li, Yong Zhang, Dongxia Zhao, Songtao Zhang, Qiong Luo, Chao Sun, Xiaochuan Zhang, Bingqian Cancer Manag Res Original Research PURPOSE: This study aimed to elucidate the biological function and upstream regulatory mechanism of CELSR1 in glioma. MATERIALS AND METHODS: We evaluated the expression of CELSR1 in glioma by TCGA_GEPIA tool, RT-qPCR, and Western blot assays. CCK-8, wound healing, and transwell invasion assays were, respectively, performed to detect the effect of CELSR1 on cell proliferation, migration, and invasion. The upstream regulatory miRNAs of CELSR1 were predicted by TargetScan and validated by luciferase activity reporter assay. RESULTS: CELSR1 is overexpressed in glioma (P<0.05). CELSR1 promoted glioma cell proliferation, migration and invasion (P<0.01). CELSR1 was a direct target of miR-199a-5p. miR199a-5p mimics significantly inhibited CELSR1 mRNA and protein expression (P<0.01). miR199a-5p mimics reversed the effects of CELSR1 on glioma cell behaviors (P<0.01). CONCLUSION: CELSR1 acts as an oncogene promoting glioma cell proliferation, migration, and invasion, which is regulated by miR199a-5p. Dove 2020-09-23 /pmc/articles/PMC7520142/ /pubmed/33061581 http://dx.doi.org/10.2147/CMAR.S258835 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Guang
Li, Yong
Zhang, Dongxia
Zhao, Songtao
Zhang, Qiong
Luo, Chao
Sun, Xiaochuan
Zhang, Bingqian
CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title_full CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title_fullStr CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title_full_unstemmed CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title_short CELSR1 Acts as an Oncogene Regulated by miR-199a-5p in Glioma
title_sort celsr1 acts as an oncogene regulated by mir-199a-5p in glioma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520142/
https://www.ncbi.nlm.nih.gov/pubmed/33061581
http://dx.doi.org/10.2147/CMAR.S258835
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