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Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis

Cancer metastasis is a common cause of failure in cancer therapy. However, over 60% of oral cancer patients present with advanced stage disease, and the five‐year survival rates of these patients decrease from 72.6% to 20% as the stage becomes more advanced. In order to manage oral cancer, identific...

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Autores principales: Huang, Hsun‐Yu, Chou, Hsiu‐Chuan, Law, Ching‐Hsuan, Chang, Wan‐Ting, Wen, Tzu‐Ning, Liao, En‐Chi, Lin, Meng‐Wei, Lin, Li‐Hsun, Wei, Yu‐Shan, Tsai, Yi‐Ting, Chen, Hsin‐Yi, Tan, Kui‐Thong, Kuo, Wen‐Hung, Ko, Mei‐Lan, Chang, Shing‐Jyh, Lee, Ying‐Ray, Chan, Hong‐Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520311/
https://www.ncbi.nlm.nih.gov/pubmed/32672400
http://dx.doi.org/10.1111/jcmm.15535
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author Huang, Hsun‐Yu
Chou, Hsiu‐Chuan
Law, Ching‐Hsuan
Chang, Wan‐Ting
Wen, Tzu‐Ning
Liao, En‐Chi
Lin, Meng‐Wei
Lin, Li‐Hsun
Wei, Yu‐Shan
Tsai, Yi‐Ting
Chen, Hsin‐Yi
Tan, Kui‐Thong
Kuo, Wen‐Hung
Ko, Mei‐Lan
Chang, Shing‐Jyh
Lee, Ying‐Ray
Chan, Hong‐Lin
author_facet Huang, Hsun‐Yu
Chou, Hsiu‐Chuan
Law, Ching‐Hsuan
Chang, Wan‐Ting
Wen, Tzu‐Ning
Liao, En‐Chi
Lin, Meng‐Wei
Lin, Li‐Hsun
Wei, Yu‐Shan
Tsai, Yi‐Ting
Chen, Hsin‐Yi
Tan, Kui‐Thong
Kuo, Wen‐Hung
Ko, Mei‐Lan
Chang, Shing‐Jyh
Lee, Ying‐Ray
Chan, Hong‐Lin
author_sort Huang, Hsun‐Yu
collection PubMed
description Cancer metastasis is a common cause of failure in cancer therapy. However, over 60% of oral cancer patients present with advanced stage disease, and the five‐year survival rates of these patients decrease from 72.6% to 20% as the stage becomes more advanced. In order to manage oral cancer, identification of metastasis biomarker and mechanism is critical. In this study, we use a pair of oral squamous cell carcinoma lines, OC3, and invasive OC3‐I5 as a model system to examine invasive mechanism and to identify potential therapeutic targets. We used two‐dimensional differential gel electrophoresis (2D‐DIGE) and matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF/TOF MS) to examine the global protein expression changes between OC3 and invasive OC3‐I5. A proteomic study reveals that invasive properties alter the expression of 101 proteins in OC3‐I5 cells comparing to OC3 cells. Further studies have used RNA interference technique to monitor the influence of progesterone receptor membrane component 1 (PGRMC1) protein in invasion and evaluate their potency in regulating invasion and the mechanism it involved. The results demonstrated that expression of epithelial‐mesenchymal transition (EMT) markers including Twist, p‐Src, Snail1, SIP1, JAM‐A, vimentin and vinculin was increased in OC3‐I5 compared to OC3 cells, whereas E‐cadherin expression was decreased in the OC3‐I5 cells. Moreover, in mouse model, PGRMC1 is shown to affect not only migration and invasion but also metastasis in vivo. Taken together, the proteomic approach allows us to identify numerous proteins, including PGRMC1, involved in invasion mechanism. Our results provide useful diagnostic markers and therapeutic candidates for the treatment of oral cancer invasion.
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spelling pubmed-75203112020-09-30 Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis Huang, Hsun‐Yu Chou, Hsiu‐Chuan Law, Ching‐Hsuan Chang, Wan‐Ting Wen, Tzu‐Ning Liao, En‐Chi Lin, Meng‐Wei Lin, Li‐Hsun Wei, Yu‐Shan Tsai, Yi‐Ting Chen, Hsin‐Yi Tan, Kui‐Thong Kuo, Wen‐Hung Ko, Mei‐Lan Chang, Shing‐Jyh Lee, Ying‐Ray Chan, Hong‐Lin J Cell Mol Med Original Articles Cancer metastasis is a common cause of failure in cancer therapy. However, over 60% of oral cancer patients present with advanced stage disease, and the five‐year survival rates of these patients decrease from 72.6% to 20% as the stage becomes more advanced. In order to manage oral cancer, identification of metastasis biomarker and mechanism is critical. In this study, we use a pair of oral squamous cell carcinoma lines, OC3, and invasive OC3‐I5 as a model system to examine invasive mechanism and to identify potential therapeutic targets. We used two‐dimensional differential gel electrophoresis (2D‐DIGE) and matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF/TOF MS) to examine the global protein expression changes between OC3 and invasive OC3‐I5. A proteomic study reveals that invasive properties alter the expression of 101 proteins in OC3‐I5 cells comparing to OC3 cells. Further studies have used RNA interference technique to monitor the influence of progesterone receptor membrane component 1 (PGRMC1) protein in invasion and evaluate their potency in regulating invasion and the mechanism it involved. The results demonstrated that expression of epithelial‐mesenchymal transition (EMT) markers including Twist, p‐Src, Snail1, SIP1, JAM‐A, vimentin and vinculin was increased in OC3‐I5 compared to OC3 cells, whereas E‐cadherin expression was decreased in the OC3‐I5 cells. Moreover, in mouse model, PGRMC1 is shown to affect not only migration and invasion but also metastasis in vivo. Taken together, the proteomic approach allows us to identify numerous proteins, including PGRMC1, involved in invasion mechanism. Our results provide useful diagnostic markers and therapeutic candidates for the treatment of oral cancer invasion. John Wiley and Sons Inc. 2020-07-16 2020-09 /pmc/articles/PMC7520311/ /pubmed/32672400 http://dx.doi.org/10.1111/jcmm.15535 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Huang, Hsun‐Yu
Chou, Hsiu‐Chuan
Law, Ching‐Hsuan
Chang, Wan‐Ting
Wen, Tzu‐Ning
Liao, En‐Chi
Lin, Meng‐Wei
Lin, Li‐Hsun
Wei, Yu‐Shan
Tsai, Yi‐Ting
Chen, Hsin‐Yi
Tan, Kui‐Thong
Kuo, Wen‐Hung
Ko, Mei‐Lan
Chang, Shing‐Jyh
Lee, Ying‐Ray
Chan, Hong‐Lin
Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title_full Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title_fullStr Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title_full_unstemmed Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title_short Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
title_sort progesterone receptor membrane component 1 is involved in oral cancer cell metastasis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520311/
https://www.ncbi.nlm.nih.gov/pubmed/32672400
http://dx.doi.org/10.1111/jcmm.15535
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