Cargando…
Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury
Low‐energy shock wave (LESW) has been recognized as a promising non‐invasive intervention to prevent the organs or tissues against ischaemia reperfusion injury (IRI), whereas its effect on kidney injury is rarely explored. To investigate the protective role of pretreatment with LESW on renal IRI in...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521246/ https://www.ncbi.nlm.nih.gov/pubmed/32761803 http://dx.doi.org/10.1111/jcmm.15678 |
_version_ | 1783587937424769024 |
---|---|
author | Liu, Jingyu Dou, Quanliang Zhou, Changcheng Zhou, Liuhua Zhao, Feng Xu, Luwei Xu, Zheng Ge, Yuzheng Wu, Ran Jia, Ruipeng |
author_facet | Liu, Jingyu Dou, Quanliang Zhou, Changcheng Zhou, Liuhua Zhao, Feng Xu, Luwei Xu, Zheng Ge, Yuzheng Wu, Ran Jia, Ruipeng |
author_sort | Liu, Jingyu |
collection | PubMed |
description | Low‐energy shock wave (LESW) has been recognized as a promising non‐invasive intervention to prevent the organs or tissues against ischaemia reperfusion injury (IRI), whereas its effect on kidney injury is rarely explored. To investigate the protective role of pretreatment with LESW on renal IRI in rats, animals were randomly divided into Sham, LESW, IRI and LESW + IRI groups. At 4, 12, 24 hours and 3 and 7 days after reperfusion, serum samples and renal tissues were harvested for performing the analysis of renal function, histopathology, immunohistochemistry, flow cytometry and Western blot, as well as enzyme‐linked immunosorbent assay. Moreover, circulating endothelial progenitor cells (EPCs) were isolated, labelled with fluorescent dye and injected by tail vein. The fluorescent signals of EPCs were detected using fluorescence microscope and in vivo imaging system to track the distribution of injected circulating EPCs. Results showed that pretreatment with LESW could significantly reduce kidney injury biomarkers, tubular damage, and cell apoptosis, and promote cell proliferation and vascularization in IRI kidneys. The renoprotective role of LESW pretreatment would be attributed to the remarkably increased EPCs in the treated kidneys, part of which were recruited from circulation through SDF‐1/CXCR7 pathway. In conclusion, pretreatment with LESW could increase the recruitment of circulating EPCs to attenuate and repair renal IRI. |
format | Online Article Text |
id | pubmed-7521246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75212462020-09-30 Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury Liu, Jingyu Dou, Quanliang Zhou, Changcheng Zhou, Liuhua Zhao, Feng Xu, Luwei Xu, Zheng Ge, Yuzheng Wu, Ran Jia, Ruipeng J Cell Mol Med Original Articles Low‐energy shock wave (LESW) has been recognized as a promising non‐invasive intervention to prevent the organs or tissues against ischaemia reperfusion injury (IRI), whereas its effect on kidney injury is rarely explored. To investigate the protective role of pretreatment with LESW on renal IRI in rats, animals were randomly divided into Sham, LESW, IRI and LESW + IRI groups. At 4, 12, 24 hours and 3 and 7 days after reperfusion, serum samples and renal tissues were harvested for performing the analysis of renal function, histopathology, immunohistochemistry, flow cytometry and Western blot, as well as enzyme‐linked immunosorbent assay. Moreover, circulating endothelial progenitor cells (EPCs) were isolated, labelled with fluorescent dye and injected by tail vein. The fluorescent signals of EPCs were detected using fluorescence microscope and in vivo imaging system to track the distribution of injected circulating EPCs. Results showed that pretreatment with LESW could significantly reduce kidney injury biomarkers, tubular damage, and cell apoptosis, and promote cell proliferation and vascularization in IRI kidneys. The renoprotective role of LESW pretreatment would be attributed to the remarkably increased EPCs in the treated kidneys, part of which were recruited from circulation through SDF‐1/CXCR7 pathway. In conclusion, pretreatment with LESW could increase the recruitment of circulating EPCs to attenuate and repair renal IRI. John Wiley and Sons Inc. 2020-08-06 2020-09 /pmc/articles/PMC7521246/ /pubmed/32761803 http://dx.doi.org/10.1111/jcmm.15678 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Liu, Jingyu Dou, Quanliang Zhou, Changcheng Zhou, Liuhua Zhao, Feng Xu, Luwei Xu, Zheng Ge, Yuzheng Wu, Ran Jia, Ruipeng Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title | Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title_full | Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title_fullStr | Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title_full_unstemmed | Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title_short | Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
title_sort | low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521246/ https://www.ncbi.nlm.nih.gov/pubmed/32761803 http://dx.doi.org/10.1111/jcmm.15678 |
work_keys_str_mv | AT liujingyu lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT douquanliang lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT zhouchangcheng lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT zhouliuhua lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT zhaofeng lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT xuluwei lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT xuzheng lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT geyuzheng lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT wuran lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury AT jiaruipeng lowenergyshockwavepretreatmentrecruitcirculatingendothelialprogenitorcellstoattenuaterenalischaemiareperfusioninjury |