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Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer

BACKGROUND: Neuropilin1 (NRP1) participates in cancer cell proliferation, migration, and metastasis as a multifunctional co‐receptor by interacting with multiple signal pathways, but few studies have addressed the precise function of NRP1 in pancreatic cancer (PACA) cells. We aimed to study whether...

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Autores principales: He, Li‐Hong, He, Yong‐Lin, Zuo, Wen‐Hang, Kang, Yue, Xue, Huan, Wang, Ling‐Yun, Zhang, Yun‐Liang, Meng, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521280/
https://www.ncbi.nlm.nih.gov/pubmed/32472711
http://dx.doi.org/10.1002/jcla.23394
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author He, Li‐Hong
He, Yong‐Lin
Zuo, Wen‐Hang
Kang, Yue
Xue, Huan
Wang, Ling‐Yun
Zhang, Yun‐Liang
Meng, Yong
author_facet He, Li‐Hong
He, Yong‐Lin
Zuo, Wen‐Hang
Kang, Yue
Xue, Huan
Wang, Ling‐Yun
Zhang, Yun‐Liang
Meng, Yong
author_sort He, Li‐Hong
collection PubMed
description BACKGROUND: Neuropilin1 (NRP1) participates in cancer cell proliferation, migration, and metastasis as a multifunctional co‐receptor by interacting with multiple signal pathways, but few studies have addressed the precise function of NRP1 in pancreatic cancer (PACA) cells. We aimed to study whether NRP1 gene silencing involved in the proliferation and migration of PACA cells in vitro. METHODS: A lentiviral vector expressing NRP1 shRNA was constructed and transfected into human PACA cells (CFPAC‐1 and PANC‐1). The expression of NRP1 protein and mRNA was detected by Western blot and quantitative real‐time polymerase chain reaction (qRT‐PCR) assay, respectively. CCK‐8 assay, wound healing assay, and transwell assay were conducted to examine the effect of NRP1 silencing on cells proliferation and migration capability. RESULTS: Results of qRT‐PCR and Western blot showed successfully established, stably transfected shRNA‐NRP1 cells in PACA cells. The proliferation capacity of PACA cells in NRP1 shRNA group was lower significantly than that in the negative control (NC) group (P < .05). The invasion and migration capability of PACA cells in NRP1 shRNA group was lower significantly than that in the NC group (P < .01). CONCLUSIONS: NRP1‐shRNA lentiviral interference vectors can effectively decrease NRP1 gene expression in PACA cells, thereby inhibiting cells proliferation and migration, which provides a basis for finding a valuable therapeutic target for PACA therapy.
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spelling pubmed-75212802020-09-30 Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer He, Li‐Hong He, Yong‐Lin Zuo, Wen‐Hang Kang, Yue Xue, Huan Wang, Ling‐Yun Zhang, Yun‐Liang Meng, Yong J Clin Lab Anal Research Articles BACKGROUND: Neuropilin1 (NRP1) participates in cancer cell proliferation, migration, and metastasis as a multifunctional co‐receptor by interacting with multiple signal pathways, but few studies have addressed the precise function of NRP1 in pancreatic cancer (PACA) cells. We aimed to study whether NRP1 gene silencing involved in the proliferation and migration of PACA cells in vitro. METHODS: A lentiviral vector expressing NRP1 shRNA was constructed and transfected into human PACA cells (CFPAC‐1 and PANC‐1). The expression of NRP1 protein and mRNA was detected by Western blot and quantitative real‐time polymerase chain reaction (qRT‐PCR) assay, respectively. CCK‐8 assay, wound healing assay, and transwell assay were conducted to examine the effect of NRP1 silencing on cells proliferation and migration capability. RESULTS: Results of qRT‐PCR and Western blot showed successfully established, stably transfected shRNA‐NRP1 cells in PACA cells. The proliferation capacity of PACA cells in NRP1 shRNA group was lower significantly than that in the negative control (NC) group (P < .05). The invasion and migration capability of PACA cells in NRP1 shRNA group was lower significantly than that in the NC group (P < .01). CONCLUSIONS: NRP1‐shRNA lentiviral interference vectors can effectively decrease NRP1 gene expression in PACA cells, thereby inhibiting cells proliferation and migration, which provides a basis for finding a valuable therapeutic target for PACA therapy. John Wiley and Sons Inc. 2020-05-30 /pmc/articles/PMC7521280/ /pubmed/32472711 http://dx.doi.org/10.1002/jcla.23394 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
He, Li‐Hong
He, Yong‐Lin
Zuo, Wen‐Hang
Kang, Yue
Xue, Huan
Wang, Ling‐Yun
Zhang, Yun‐Liang
Meng, Yong
Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title_full Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title_fullStr Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title_full_unstemmed Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title_short Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
title_sort neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521280/
https://www.ncbi.nlm.nih.gov/pubmed/32472711
http://dx.doi.org/10.1002/jcla.23394
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