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PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury
Inflammatory cell infiltration contributes to the pathogenesis of acute respiratory distress syndrome (ARDS). Protectin DX (PDX), an endogenous lipid mediator, shows anti‐inflammatory and proresolution bioactions. In vivo, the mice were intraperitoneally injected with PDX (0.1 µg/mouse) after intrat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521295/ https://www.ncbi.nlm.nih.gov/pubmed/32735065 http://dx.doi.org/10.1111/jcmm.15679 |
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author | Ye, Yang Zhang, Hua‐Wei Mei, Hong‐Xia Xu, Hao‐Ran Xiang, Shu‐Yang Yang, Qian Zheng, Sheng‐Xing Gao Smith, Fang Jin, Sheng‐Wei Wang, Qian |
author_facet | Ye, Yang Zhang, Hua‐Wei Mei, Hong‐Xia Xu, Hao‐Ran Xiang, Shu‐Yang Yang, Qian Zheng, Sheng‐Xing Gao Smith, Fang Jin, Sheng‐Wei Wang, Qian |
author_sort | Ye, Yang |
collection | PubMed |
description | Inflammatory cell infiltration contributes to the pathogenesis of acute respiratory distress syndrome (ARDS). Protectin DX (PDX), an endogenous lipid mediator, shows anti‐inflammatory and proresolution bioactions. In vivo, the mice were intraperitoneally injected with PDX (0.1 µg/mouse) after intratracheal (1 mg/kg) or intraperitoneal (10 mg/kg) LPS administration. Flow cytometry was used to measure inflammatory cell numbers. Clodronate liposomes were used to deplete resident macrophages. RT‐PCR, and ELISA was used to measure MIP‐2, MCP‐1, TNF‐α and MMP9 levels. In vitro, sorted neutrophils, resident and recruited macrophages (1 × 10(6)) were cultured with 1 μg/mL LPS and/or 100 nmol/L PDX to assess the chemokine receptor expression. PDX attenuated LPS‐induced lung injury via inhibiting recruited macrophage and neutrophil recruitment through repressing resident macrophage MCP‐1, MIP‐2 expression and release, respectively. Finally, PDX inhibition of neutrophil infiltration and transmembrane was associated with TNF‐α/MIP‐2/MMP9 signalling pathway. These data suggest that PDX attenuates LPS‐stimulated lung injury via reduction of the inflammatory cell recruitment mediated via resident macrophages. |
format | Online Article Text |
id | pubmed-7521295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75212952020-10-02 PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury Ye, Yang Zhang, Hua‐Wei Mei, Hong‐Xia Xu, Hao‐Ran Xiang, Shu‐Yang Yang, Qian Zheng, Sheng‐Xing Gao Smith, Fang Jin, Sheng‐Wei Wang, Qian J Cell Mol Med Original Articles Inflammatory cell infiltration contributes to the pathogenesis of acute respiratory distress syndrome (ARDS). Protectin DX (PDX), an endogenous lipid mediator, shows anti‐inflammatory and proresolution bioactions. In vivo, the mice were intraperitoneally injected with PDX (0.1 µg/mouse) after intratracheal (1 mg/kg) or intraperitoneal (10 mg/kg) LPS administration. Flow cytometry was used to measure inflammatory cell numbers. Clodronate liposomes were used to deplete resident macrophages. RT‐PCR, and ELISA was used to measure MIP‐2, MCP‐1, TNF‐α and MMP9 levels. In vitro, sorted neutrophils, resident and recruited macrophages (1 × 10(6)) were cultured with 1 μg/mL LPS and/or 100 nmol/L PDX to assess the chemokine receptor expression. PDX attenuated LPS‐induced lung injury via inhibiting recruited macrophage and neutrophil recruitment through repressing resident macrophage MCP‐1, MIP‐2 expression and release, respectively. Finally, PDX inhibition of neutrophil infiltration and transmembrane was associated with TNF‐α/MIP‐2/MMP9 signalling pathway. These data suggest that PDX attenuates LPS‐stimulated lung injury via reduction of the inflammatory cell recruitment mediated via resident macrophages. John Wiley and Sons Inc. 2020-07-31 2020-09 /pmc/articles/PMC7521295/ /pubmed/32735065 http://dx.doi.org/10.1111/jcmm.15679 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ye, Yang Zhang, Hua‐Wei Mei, Hong‐Xia Xu, Hao‐Ran Xiang, Shu‐Yang Yang, Qian Zheng, Sheng‐Xing Gao Smith, Fang Jin, Sheng‐Wei Wang, Qian PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title | PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title_full | PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title_fullStr | PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title_full_unstemmed | PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title_short | PDX regulates inflammatory cell infiltration via resident macrophage in LPS‐induced lung injury |
title_sort | pdx regulates inflammatory cell infiltration via resident macrophage in lps‐induced lung injury |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521295/ https://www.ncbi.nlm.nih.gov/pubmed/32735065 http://dx.doi.org/10.1111/jcmm.15679 |
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