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Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes

Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to d...

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Autores principales: Pan, Yuhong, Cheng, Anchun, Zhang, Xingcui, Wang, Mingshu, Chen, Shun, Zhu, Dekang, Liu, Mafeng, Zhao, Xinxin, Yang, Qiao, Wu, Ying, Huang, Juan, Zhang, Shaqiu, Mao, Sai, Ou, Xumin, Gao, Qun, Yu, Yanling, Liu, Yunya, Zhang, Ling, Yin, Zhongqiong, Jing, Bo, Tian, Bin, Pan, Leichang, Rehman, Mujeeb Ur, Chen, Xiaoyue, Jia, Renyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521532/
https://www.ncbi.nlm.nih.gov/pubmed/32897243
http://dx.doi.org/10.18632/aging.103759
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author Pan, Yuhong
Cheng, Anchun
Zhang, Xingcui
Wang, Mingshu
Chen, Shun
Zhu, Dekang
Liu, Mafeng
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Huang, Juan
Zhang, Shaqiu
Mao, Sai
Ou, Xumin
Gao, Qun
Yu, Yanling
Liu, Yunya
Zhang, Ling
Yin, Zhongqiong
Jing, Bo
Tian, Bin
Pan, Leichang
Rehman, Mujeeb Ur
Chen, Xiaoyue
Jia, Renyong
author_facet Pan, Yuhong
Cheng, Anchun
Zhang, Xingcui
Wang, Mingshu
Chen, Shun
Zhu, Dekang
Liu, Mafeng
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Huang, Juan
Zhang, Shaqiu
Mao, Sai
Ou, Xumin
Gao, Qun
Yu, Yanling
Liu, Yunya
Zhang, Ling
Yin, Zhongqiong
Jing, Bo
Tian, Bin
Pan, Leichang
Rehman, Mujeeb Ur
Chen, Xiaoyue
Jia, Renyong
author_sort Pan, Yuhong
collection PubMed
description Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to detect the global gene expression in DEFs at various time points after DTMUV infection. We identified 326 genes altered significantly at all time points, and these genes were dynamically enriched in multifarious biological processes, including apoptosis, innate immune response, DNA replication, cell cycle arrest and DNA repair. Next, the results showed that apoptosis was induced and the proportion of apoptosis increased with time, and pro-apoptotic molecules caspases were activated. The RNA-seq data analysis further revealed that most pro-apoptosis and anti-apoptosis genes were early continually responsive, and the genes involved in both intrinsic and extrinsic apoptotic pathways were initiated. Further, the considerably enriched immune-relevant pathways were involved in apoptosis process, and protein-protein interactions (PPIs) analysis showed that IL6, STAT1, TNFAIP3, CFLAR and PTGS2 may be key regulators of DEFs apoptosis. In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy.
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spelling pubmed-75215322020-10-02 Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes Pan, Yuhong Cheng, Anchun Zhang, Xingcui Wang, Mingshu Chen, Shun Zhu, Dekang Liu, Mafeng Zhao, Xinxin Yang, Qiao Wu, Ying Huang, Juan Zhang, Shaqiu Mao, Sai Ou, Xumin Gao, Qun Yu, Yanling Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Tian, Bin Pan, Leichang Rehman, Mujeeb Ur Chen, Xiaoyue Jia, Renyong Aging (Albany NY) Research Paper Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to detect the global gene expression in DEFs at various time points after DTMUV infection. We identified 326 genes altered significantly at all time points, and these genes were dynamically enriched in multifarious biological processes, including apoptosis, innate immune response, DNA replication, cell cycle arrest and DNA repair. Next, the results showed that apoptosis was induced and the proportion of apoptosis increased with time, and pro-apoptotic molecules caspases were activated. The RNA-seq data analysis further revealed that most pro-apoptosis and anti-apoptosis genes were early continually responsive, and the genes involved in both intrinsic and extrinsic apoptotic pathways were initiated. Further, the considerably enriched immune-relevant pathways were involved in apoptosis process, and protein-protein interactions (PPIs) analysis showed that IL6, STAT1, TNFAIP3, CFLAR and PTGS2 may be key regulators of DEFs apoptosis. In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy. Impact Journals 2020-09-07 /pmc/articles/PMC7521532/ /pubmed/32897243 http://dx.doi.org/10.18632/aging.103759 Text en Copyright: © 2020 Pan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Pan, Yuhong
Cheng, Anchun
Zhang, Xingcui
Wang, Mingshu
Chen, Shun
Zhu, Dekang
Liu, Mafeng
Zhao, Xinxin
Yang, Qiao
Wu, Ying
Huang, Juan
Zhang, Shaqiu
Mao, Sai
Ou, Xumin
Gao, Qun
Yu, Yanling
Liu, Yunya
Zhang, Ling
Yin, Zhongqiong
Jing, Bo
Tian, Bin
Pan, Leichang
Rehman, Mujeeb Ur
Chen, Xiaoyue
Jia, Renyong
Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title_full Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title_fullStr Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title_full_unstemmed Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title_short Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
title_sort transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521532/
https://www.ncbi.nlm.nih.gov/pubmed/32897243
http://dx.doi.org/10.18632/aging.103759
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