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Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes
Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to d...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521532/ https://www.ncbi.nlm.nih.gov/pubmed/32897243 http://dx.doi.org/10.18632/aging.103759 |
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author | Pan, Yuhong Cheng, Anchun Zhang, Xingcui Wang, Mingshu Chen, Shun Zhu, Dekang Liu, Mafeng Zhao, Xinxin Yang, Qiao Wu, Ying Huang, Juan Zhang, Shaqiu Mao, Sai Ou, Xumin Gao, Qun Yu, Yanling Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Tian, Bin Pan, Leichang Rehman, Mujeeb Ur Chen, Xiaoyue Jia, Renyong |
author_facet | Pan, Yuhong Cheng, Anchun Zhang, Xingcui Wang, Mingshu Chen, Shun Zhu, Dekang Liu, Mafeng Zhao, Xinxin Yang, Qiao Wu, Ying Huang, Juan Zhang, Shaqiu Mao, Sai Ou, Xumin Gao, Qun Yu, Yanling Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Tian, Bin Pan, Leichang Rehman, Mujeeb Ur Chen, Xiaoyue Jia, Renyong |
author_sort | Pan, Yuhong |
collection | PubMed |
description | Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to detect the global gene expression in DEFs at various time points after DTMUV infection. We identified 326 genes altered significantly at all time points, and these genes were dynamically enriched in multifarious biological processes, including apoptosis, innate immune response, DNA replication, cell cycle arrest and DNA repair. Next, the results showed that apoptosis was induced and the proportion of apoptosis increased with time, and pro-apoptotic molecules caspases were activated. The RNA-seq data analysis further revealed that most pro-apoptosis and anti-apoptosis genes were early continually responsive, and the genes involved in both intrinsic and extrinsic apoptotic pathways were initiated. Further, the considerably enriched immune-relevant pathways were involved in apoptosis process, and protein-protein interactions (PPIs) analysis showed that IL6, STAT1, TNFAIP3, CFLAR and PTGS2 may be key regulators of DEFs apoptosis. In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy. |
format | Online Article Text |
id | pubmed-7521532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-75215322020-10-02 Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes Pan, Yuhong Cheng, Anchun Zhang, Xingcui Wang, Mingshu Chen, Shun Zhu, Dekang Liu, Mafeng Zhao, Xinxin Yang, Qiao Wu, Ying Huang, Juan Zhang, Shaqiu Mao, Sai Ou, Xumin Gao, Qun Yu, Yanling Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Tian, Bin Pan, Leichang Rehman, Mujeeb Ur Chen, Xiaoyue Jia, Renyong Aging (Albany NY) Research Paper Duck Tembusu virus (DTMUV) is an emerging pathogenic flavivirus that has caused enormous economic losses in Southeast Asia. However, the pathogenic mechanism and host’s responses after DTMUV infection remain poorly understood. During this study, total mRNA sequencing (RNA-Seq) analysis was used to detect the global gene expression in DEFs at various time points after DTMUV infection. We identified 326 genes altered significantly at all time points, and these genes were dynamically enriched in multifarious biological processes, including apoptosis, innate immune response, DNA replication, cell cycle arrest and DNA repair. Next, the results showed that apoptosis was induced and the proportion of apoptosis increased with time, and pro-apoptotic molecules caspases were activated. The RNA-seq data analysis further revealed that most pro-apoptosis and anti-apoptosis genes were early continually responsive, and the genes involved in both intrinsic and extrinsic apoptotic pathways were initiated. Further, the considerably enriched immune-relevant pathways were involved in apoptosis process, and protein-protein interactions (PPIs) analysis showed that IL6, STAT1, TNFAIP3, CFLAR and PTGS2 may be key regulators of DEFs apoptosis. In conclusion, this study not only contributes to understanding the underlying mechanism of DEFs infection with DTMUV, but also provides new insights into targets screening for antiviral therapy. Impact Journals 2020-09-07 /pmc/articles/PMC7521532/ /pubmed/32897243 http://dx.doi.org/10.18632/aging.103759 Text en Copyright: © 2020 Pan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Pan, Yuhong Cheng, Anchun Zhang, Xingcui Wang, Mingshu Chen, Shun Zhu, Dekang Liu, Mafeng Zhao, Xinxin Yang, Qiao Wu, Ying Huang, Juan Zhang, Shaqiu Mao, Sai Ou, Xumin Gao, Qun Yu, Yanling Liu, Yunya Zhang, Ling Yin, Zhongqiong Jing, Bo Tian, Bin Pan, Leichang Rehman, Mujeeb Ur Chen, Xiaoyue Jia, Renyong Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title | Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title_full | Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title_fullStr | Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title_full_unstemmed | Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title_short | Transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
title_sort | transcriptome analysis of duck embryo fibroblasts for the dynamic response to duck tembusu virus infection and dual regulation of apoptosis genes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521532/ https://www.ncbi.nlm.nih.gov/pubmed/32897243 http://dx.doi.org/10.18632/aging.103759 |
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