Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use

The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined PO...

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Detalles Bibliográficos
Autores principales: Ramadurgum, Prerana, Hulleman, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521665/
https://www.ncbi.nlm.nih.gov/pubmed/32995752
http://dx.doi.org/10.1016/j.xpro.2020.100069
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author Ramadurgum, Prerana
Hulleman, John D.
author_facet Ramadurgum, Prerana
Hulleman, John D.
author_sort Ramadurgum, Prerana
collection PubMed
description The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020).
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spelling pubmed-75216652020-09-28 Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use Ramadurgum, Prerana Hulleman, John D. STAR Protoc Protocol The use of destabilizing domains (DDs) to conditionally control the abundance of a protein of interest (POI) through a small-molecule stabilizer has gained increasing traction both in vitro and in vivo. Yet there are specific considerations for the development and accurate control of user-defined POIs via DDs, as well as the identification of novel (and potentially synergistic) small-molecule stabilizers. Here, we describe a platform for achieving these goals. For complete details on the use and execution of this protocol, please refer to Ramadurgum et al. (2020). Elsevier 2020-07-24 /pmc/articles/PMC7521665/ /pubmed/32995752 http://dx.doi.org/10.1016/j.xpro.2020.100069 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ramadurgum, Prerana
Hulleman, John D.
Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title_full Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title_fullStr Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title_full_unstemmed Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title_short Protocol for Designing Small-Molecule-Regulated Destabilizing Domains for In Vitro Use
title_sort protocol for designing small-molecule-regulated destabilizing domains for in vitro use
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521665/
https://www.ncbi.nlm.nih.gov/pubmed/32995752
http://dx.doi.org/10.1016/j.xpro.2020.100069
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