Cargando…

Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

PURPOSE: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cance...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Duo, Zhao, Hongyu, Zeng, Peng, Song, Jinyun, Yang, Yiqiong, Gu, Xuefeng, Ji, Qinghua, Zhao, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Gastric Cancer Association 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521983/
https://www.ncbi.nlm.nih.gov/pubmed/33024586
http://dx.doi.org/10.5230/jgc.2020.20.e28
_version_ 1783588084548370432
author Shen, Duo
Zhao, Hongyu
Zeng, Peng
Song, Jinyun
Yang, Yiqiong
Gu, Xuefeng
Ji, Qinghua
Zhao, Wei
author_facet Shen, Duo
Zhao, Hongyu
Zeng, Peng
Song, Jinyun
Yang, Yiqiong
Gu, Xuefeng
Ji, Qinghua
Zhao, Wei
author_sort Shen, Duo
collection PubMed
description PURPOSE: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. MATERIALS AND METHODS: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. RESULTS: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. CONCLUSIONS: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.
format Online
Article
Text
id pubmed-7521983
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Korean Gastric Cancer Association
record_format MEDLINE/PubMed
spelling pubmed-75219832020-10-05 Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression Shen, Duo Zhao, Hongyu Zeng, Peng Song, Jinyun Yang, Yiqiong Gu, Xuefeng Ji, Qinghua Zhao, Wei J Gastric Cancer Original Article PURPOSE: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. MATERIALS AND METHODS: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. RESULTS: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. CONCLUSIONS: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism. The Korean Gastric Cancer Association 2020-09 2020-09-14 /pmc/articles/PMC7521983/ /pubmed/33024586 http://dx.doi.org/10.5230/jgc.2020.20.e28 Text en Copyright © 2020. Korean Gastric Cancer Association https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Shen, Duo
Zhao, Hongyu
Zeng, Peng
Song, Jinyun
Yang, Yiqiong
Gu, Xuefeng
Ji, Qinghua
Zhao, Wei
Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title_full Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title_fullStr Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title_full_unstemmed Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title_short Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression
title_sort circular rna hsa_circ_0005556 accelerates gastric cancer progression by sponging mir-4270 to increase mmp19 expression
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521983/
https://www.ncbi.nlm.nih.gov/pubmed/33024586
http://dx.doi.org/10.5230/jgc.2020.20.e28
work_keys_str_mv AT shenduo circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT zhaohongyu circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT zengpeng circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT songjinyun circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT yangyiqiong circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT guxuefeng circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT jiqinghua circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression
AT zhaowei circularrnahsacirc0005556acceleratesgastriccancerprogressionbyspongingmir4270toincreasemmp19expression