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De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522117/ https://www.ncbi.nlm.nih.gov/pubmed/33014707 http://dx.doi.org/10.1016/j.mec.2020.e00146 |
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author | He, Qin Szczepańska, Patrycja Yuzbashev, Tigran Lazar, Zbigniew Ledesma-Amaro, Rodrigo |
author_facet | He, Qin Szczepańska, Patrycja Yuzbashev, Tigran Lazar, Zbigniew Ledesma-Amaro, Rodrigo |
author_sort | He, Qin |
collection | PubMed |
description | Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol. |
format | Online Article Text |
id | pubmed-7522117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75221172020-10-02 De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica He, Qin Szczepańska, Patrycja Yuzbashev, Tigran Lazar, Zbigniew Ledesma-Amaro, Rodrigo Metab Eng Commun Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol. Elsevier 2020-09-19 /pmc/articles/PMC7522117/ /pubmed/33014707 http://dx.doi.org/10.1016/j.mec.2020.e00146 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro He, Qin Szczepańska, Patrycja Yuzbashev, Tigran Lazar, Zbigniew Ledesma-Amaro, Rodrigo De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title | De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title_full | De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title_fullStr | De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title_full_unstemmed | De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title_short | De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica |
title_sort | de novo production of resveratrol from glycerol by engineering different metabolic pathways in yarrowia lipolytica |
topic | Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522117/ https://www.ncbi.nlm.nih.gov/pubmed/33014707 http://dx.doi.org/10.1016/j.mec.2020.e00146 |
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