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De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratr...

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Autores principales: He, Qin, Szczepańska, Patrycja, Yuzbashev, Tigran, Lazar, Zbigniew, Ledesma-Amaro, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522117/
https://www.ncbi.nlm.nih.gov/pubmed/33014707
http://dx.doi.org/10.1016/j.mec.2020.e00146
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author He, Qin
Szczepańska, Patrycja
Yuzbashev, Tigran
Lazar, Zbigniew
Ledesma-Amaro, Rodrigo
author_facet He, Qin
Szczepańska, Patrycja
Yuzbashev, Tigran
Lazar, Zbigniew
Ledesma-Amaro, Rodrigo
author_sort He, Qin
collection PubMed
description Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 ​g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 ​g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 ​g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol.
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spelling pubmed-75221172020-10-02 De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica He, Qin Szczepańska, Patrycja Yuzbashev, Tigran Lazar, Zbigniew Ledesma-Amaro, Rodrigo Metab Eng Commun Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 ​g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 ​g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 ​g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol. Elsevier 2020-09-19 /pmc/articles/PMC7522117/ /pubmed/33014707 http://dx.doi.org/10.1016/j.mec.2020.e00146 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro
He, Qin
Szczepańska, Patrycja
Yuzbashev, Tigran
Lazar, Zbigniew
Ledesma-Amaro, Rodrigo
De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title_full De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title_fullStr De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title_full_unstemmed De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title_short De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
title_sort de novo production of resveratrol from glycerol by engineering different metabolic pathways in yarrowia lipolytica
topic Special issue on Model chassis cells edited by Long Liu and Rodrigo Ledesma- Amaro
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522117/
https://www.ncbi.nlm.nih.gov/pubmed/33014707
http://dx.doi.org/10.1016/j.mec.2020.e00146
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