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Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities
Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues (brain, heart, kidney, liver, lung, ovary and spl...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522264/ https://www.ncbi.nlm.nih.gov/pubmed/32985598 http://dx.doi.org/10.1038/s41598-020-72959-8 |
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author | Heffner, Kelley Hizal, Deniz Baycin Majewska, Natalia I. Kumar, Swetha Dhara, Venkata Gayatri Zhu, Jie Bowen, Michael Hatton, Diane Yerganian, George Yerganian, Athena O’Meally, Robert Cole, Robert Betenbaugh, Michael |
author_facet | Heffner, Kelley Hizal, Deniz Baycin Majewska, Natalia I. Kumar, Swetha Dhara, Venkata Gayatri Zhu, Jie Bowen, Michael Hatton, Diane Yerganian, George Yerganian, Athena O’Meally, Robert Cole, Robert Betenbaugh, Michael |
author_sort | Heffner, Kelley |
collection | PubMed |
description | Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues (brain, heart, kidney, liver, lung, ovary and spleen) by tandem mass tag (TMT) labeling followed by mass spectrometry, providing a comprehensive hamster tissue and cell line proteomics atlas. Of the 8470 unique proteins identified, high similarity was observed between CHO-S and CHO DG44 and included increases in proteins involved in DNA replication, cell cycle, RNA processing, and chromosome processing. Alternatively, gene ontology and pathway analysis in tissues indicated increased protein intensities related to important tissue functionalities. Proteins enriched in the brain included those involved in acidic amino acid metabolism, Golgi apparatus, and ion and phospholipid transport. The lung showed enrichment in proteins involved in BCAA catabolism, ROS metabolism, vesicle trafficking, and lipid synthesis while the ovary exhibited enrichments in extracellular matrix and adhesion proteins. The heart proteome included vasoconstriction, complement activation, and lipoprotein metabolism enrichments. These detailed comparisons of CHO cell lines and hamster tissues will enhance understanding of the relationship between proteins and tissue function and pinpoint potential pathways of biotechnological relevance for future cell engineering. |
format | Online Article Text |
id | pubmed-7522264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75222642020-09-29 Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities Heffner, Kelley Hizal, Deniz Baycin Majewska, Natalia I. Kumar, Swetha Dhara, Venkata Gayatri Zhu, Jie Bowen, Michael Hatton, Diane Yerganian, George Yerganian, Athena O’Meally, Robert Cole, Robert Betenbaugh, Michael Sci Rep Article Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues (brain, heart, kidney, liver, lung, ovary and spleen) by tandem mass tag (TMT) labeling followed by mass spectrometry, providing a comprehensive hamster tissue and cell line proteomics atlas. Of the 8470 unique proteins identified, high similarity was observed between CHO-S and CHO DG44 and included increases in proteins involved in DNA replication, cell cycle, RNA processing, and chromosome processing. Alternatively, gene ontology and pathway analysis in tissues indicated increased protein intensities related to important tissue functionalities. Proteins enriched in the brain included those involved in acidic amino acid metabolism, Golgi apparatus, and ion and phospholipid transport. The lung showed enrichment in proteins involved in BCAA catabolism, ROS metabolism, vesicle trafficking, and lipid synthesis while the ovary exhibited enrichments in extracellular matrix and adhesion proteins. The heart proteome included vasoconstriction, complement activation, and lipoprotein metabolism enrichments. These detailed comparisons of CHO cell lines and hamster tissues will enhance understanding of the relationship between proteins and tissue function and pinpoint potential pathways of biotechnological relevance for future cell engineering. Nature Publishing Group UK 2020-09-28 /pmc/articles/PMC7522264/ /pubmed/32985598 http://dx.doi.org/10.1038/s41598-020-72959-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Heffner, Kelley Hizal, Deniz Baycin Majewska, Natalia I. Kumar, Swetha Dhara, Venkata Gayatri Zhu, Jie Bowen, Michael Hatton, Diane Yerganian, George Yerganian, Athena O’Meally, Robert Cole, Robert Betenbaugh, Michael Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title | Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title_full | Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title_fullStr | Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title_full_unstemmed | Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title_short | Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
title_sort | expanded chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522264/ https://www.ncbi.nlm.nih.gov/pubmed/32985598 http://dx.doi.org/10.1038/s41598-020-72959-8 |
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