Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients

Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free...

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Autores principales: Steiner, Daniel J., Cognetti, John S., Luta, Ethan P., Klose, Alanna M., Bucukovski, Joseph, Bryan, Michael R., Schmuke, Jon J., Nguyen-Contant, Phuong, Sangster, Mark Y., Topham, David J., Miller, Benjamin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522665/
https://www.ncbi.nlm.nih.gov/pubmed/33007615
http://dx.doi.org/10.1016/j.bios.2020.112643
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author Steiner, Daniel J.
Cognetti, John S.
Luta, Ethan P.
Klose, Alanna M.
Bucukovski, Joseph
Bryan, Michael R.
Schmuke, Jon J.
Nguyen-Contant, Phuong
Sangster, Mark Y.
Topham, David J.
Miller, Benjamin L.
author_facet Steiner, Daniel J.
Cognetti, John S.
Luta, Ethan P.
Klose, Alanna M.
Bucukovski, Joseph
Bryan, Michael R.
Schmuke, Jon J.
Nguyen-Contant, Phuong
Sangster, Mark Y.
Topham, David J.
Miller, Benjamin L.
author_sort Steiner, Daniel J.
collection PubMed
description Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses.
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spelling pubmed-75226652020-09-29 Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients Steiner, Daniel J. Cognetti, John S. Luta, Ethan P. Klose, Alanna M. Bucukovski, Joseph Bryan, Michael R. Schmuke, Jon J. Nguyen-Contant, Phuong Sangster, Mark Y. Topham, David J. Miller, Benjamin L. Biosens Bioelectron Article Detection of antibodies to upper respiratory pathogens is critical to surveillance, assessment of the immune status of individuals, vaccine development, and basic biology. The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. We report a multiplex label-free antigen microarray on the Arrayed Imaging Reflectometry (AIR) platform for detection of antibodies to SARS-CoV-2, SARS-CoV-1, MERS, three circulating coronavirus strains (HKU1, 229E, OC43) and three strains of influenza. We find that the array is readily able to distinguish uninfected from convalescent COVID-19 subjects, and provides quantitative information about total Ig, as well as IgG- and IgM-specific responses. Elsevier B.V. 2020-12-01 2020-09-21 /pmc/articles/PMC7522665/ /pubmed/33007615 http://dx.doi.org/10.1016/j.bios.2020.112643 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Steiner, Daniel J.
Cognetti, John S.
Luta, Ethan P.
Klose, Alanna M.
Bucukovski, Joseph
Bryan, Michael R.
Schmuke, Jon J.
Nguyen-Contant, Phuong
Sangster, Mark Y.
Topham, David J.
Miller, Benjamin L.
Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title_full Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title_fullStr Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title_full_unstemmed Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title_short Array-based analysis of SARS-CoV-2, other coronaviruses, and influenza antibodies in convalescent COVID-19 patients
title_sort array-based analysis of sars-cov-2, other coronaviruses, and influenza antibodies in convalescent covid-19 patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7522665/
https://www.ncbi.nlm.nih.gov/pubmed/33007615
http://dx.doi.org/10.1016/j.bios.2020.112643
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